Zhao Jiayuan, Chi Yuanlong, Xu Yingchao, Jia Dongying, Yao Kai
College of Light Industry, Textile & Food Engineering, Sichuan University, 610065, Chengdu, Sichuan, P. R. China.
PLoS One. 2016 Nov 29;11(11):e0166796. doi: 10.1371/journal.pone.0166796. eCollection 2016.
The degradation efficiency of organic contaminants and their associated metabolites by co-culture of microbes is mainly limited by toxic intermediates from co-metabolic degradation. In this study, we investigated the degradation of β-cypermethrin (β-CY) and 3-phenoxybenzoic acid (3-PBA) by co-culture of Bacillus licheniformis B-1 and Aspergillus oryzae M-4, as well as the influences of β-CY and 3-PBA metabolites on their degradation and the growth of strains B-1 and M-4. Our results indicated that 100 mg/L β-CY was degraded by 78.85%, and 3-PBA concentration was 0.05 mg/L after 72 h. Compared with using only strain B-1, the half-life (t1/2) of β-CY by using the two strains together was shortened from 84.53 h to 38.54 h, and the yield coefficient of 3-PBA was decreased from 0.846 to 0.001. At 100 mg/L of 3-PBA and gallic acid, β-CY and 3-PBA degradation were only 17.68% and 40.45%, respectively. As the toxic intermediate derived from co-metabolic degradation of β-CY by strain B-1, 3-PBA was efficiently degraded by strain M-4, and gallic acid, as the toxic intermediate from co-metabolic degradation of 3-PBA by strain M-4, was efficiently degraded by strain B-1. These results provided a promising approach for efficient biodegradation of β-CY and 3-PBA.
微生物共培养对有机污染物及其相关代谢产物的降解效率主要受共代谢降解产生的有毒中间体的限制。在本研究中,我们研究了地衣芽孢杆菌B-1和米曲霉M-4共培养对高效氯氰菊酯(β-CY)和3-苯氧基苯甲酸(3-PBA)的降解情况,以及β-CY和3-PBA代谢产物对其降解及菌株B-1和M-4生长的影响。我们的结果表明,72小时后100mg/L的β-CY降解了78.85%,3-PBA浓度为0.05mg/L。与仅使用菌株B-1相比,两种菌株共同使用时β-CY的半衰期(t1/2)从84.53小时缩短至38.54小时,3-PBA的产率系数从0.846降至0.001。在100mg/L的3-PBA和没食子酸存在下,β-CY和3-PBA的降解率分别仅为17.68%和40.45%。作为菌株B-1对β-CY共代谢降解产生的有毒中间体,3-PBA被菌株M-4有效降解,而没食子酸作为菌株M-4对3-PBA共代谢降解产生的有毒中间体,被菌株B-1有效降解。这些结果为β-CY和3-PBA的高效生物降解提供了一种有前景的方法。