Theoretical considerations in the estimation of rumen fractional outflow rate from various sampling sites in the digestive tract.

The digesta markers 103ruthenium phenanthroline (Ru-P) and 51Cr-EDTA were continuously infused into the rumen of young lambs offered clover hay ad lib. indoors or grazing pure species swards of either lucerne (Medicago sativa), white clover (Trifolium repens), ryegrass (Lolium perenne) or prairie grass (Bromus catharticus). Following the cessation of infusion the fractional outflow rate (FOR) of markers from the rumen was estimated from the rate of decline of marker concentration in rumen and abomasal digesta (Expt 1), abomasal digesta and faeces (Expt 2) and duodenal digesta and faeces (Expt 3). Estimates of FOR from the rumen were similar when based on rumen and abomasal sampling but estimates from abomasal and duodenal sampling were significantly higher than those from faeces. A simulation of a two-compartment model with variable FOR was performed to calculate faecal excretion curves following continuous marker infusion or the administration of a single marker dose into the rumen. Analysis of the descending portion of these theoretical faecal excretion curves yielded values of FOR from the rumen which differed from the values entered in the equations. The magnitude of the difference depended on the ratio of the values of FOR from the caecum and rumen entered in the equation. This suggested that the experimental variation observed in these young lambs was caused by the high FOR from the rumen in relation to the FOR from the caecum-large intestine.