Aka Juliette A, Calvo Ezequiel-Luis, Lin Sheng-Xiang
Laboratory of Molecular Endocrinology and Oncology, Centre Hospitalier Universitaire de Québec Research Centre (CHUQ, CHUL) and Department of Molecular Medicine, Laval University, 2705 boulevard Laurier, Québec G1V 4G2, Canada.
Data Brief. 2016 Nov 9;9:1000-1012. doi: 10.1016/j.dib.2016.11.010. eCollection 2016 Dec.
The data presented here are related to the research article entitled "Estradiol-independent modulation of breast cancer transcript profile by 17beta-hydroxysteroid dehydrogenase type 1" (J.A. Aka, E.L. Calvo, S.X. Lin, 2016) [1]. We evaluated the effect of the steroidal enzyme 17β-HSD1 and its product, the estrogenic hormone 17-beta-estradiol (E2), on gene transcription profile of breast cancer cells. RNA interference technique was used to knock down the 17β-HSD1 gene () in the hormone-dependent breast cancer cell line T47D in steroid-deprived medium. Transfected cells were subsequently treated with E2, and microarray analyses (with three contrasts) were used to investigate (i) the effect of 17β-HSD1 expression on breast cancer cell transcript profile in steroid-deprived condition, (ii) the effect of E2 on breast cancer gene expression and (iii) if E2 affects gene regulation by 17β-HSD1. Functional enrichments of the differentially expressed genes were assessed using Ingenuity Pathway Analysis (IPA). Here, we showed data on 140 genes that are induced or repressed 1.5 time or higher ( < 0.05) in the silenced and E2-treated T47D cells revealed by microarray analysis, and presented the 14 functional terms found in the cancer and in the cell death and survival categories revealed by the IPA biological function analysis. Data on IPA Canonical Pathway and network analyses is also presented. Further discussion on gene regulation by 17β-HSD1 and E2 is provided in the accompanying publication [1].
此处呈现的数据与题为《17β-羟基类固醇脱氢酶1对乳腺癌转录谱的非雌激素依赖性调节》（J.A. 阿卡、E.L. 卡尔沃、S.X. 林，2016年）[1]的研究论文相关。我们评估了甾体酶17β-HSD1及其产物雌激素17-β-雌二醇（E2）对乳腺癌细胞基因转录谱的影响。在缺乏类固醇的培养基中，利用RNA干扰技术敲低激素依赖性乳腺癌细胞系T47D中的17β-HSD1基因（）。随后用E2处理转染后的细胞，并使用微阵列分析（进行三次对比）来研究：（i）在缺乏类固醇的条件下，17β-HSD1表达对乳腺癌细胞转录谱的影响；（ii）E2对乳腺癌基因表达的影响；以及（iii）E2是否影响17β-HSD1对基因的调控。使用 Ingenuity 通路分析（IPA）评估差异表达基因的功能富集情况。在此，我们展示了微阵列分析揭示的在沉默且经E2处理的T47D细胞中诱导或抑制1.5倍及以上（<0.05）的140个基因的数据，并呈现了IPA生物学功能分析揭示的在癌症以及细胞死亡和存活类别中发现的14个功能术语。还展示了关于IPA经典通路和网络分析的数据。随附出版物[1]中提供了关于17β-HSD1和E2对基因调控的进一步讨论。
