Mulloy B, Lever R, Page C P
Sackler Institute of Pulmonary Pharmacology, Institute for Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford St, London, SE1 9NN, UK.
1 UCL School of Pharmacy, Brunswick Square, London, WC1N 1AX, UK.
Glycoconj J. 2017 Jun;34(3):351-361. doi: 10.1007/s10719-016-9749-0. Epub 2016 Nov 30.
Mast cells contain granules packed with a mixture of proteins that are released on degranulation. The proteoglycan serglycin carries an array of glycosaminoglycan (GAG) side chains, sometimes heparin, sometimes chondroitin or dermatan sulphate. Tight packing of granule proteins is dependent on the presence of serglycin carrying these GAGs. The GAGs of mast cells were most intensively studied in the 1970s and 1980s, and though something is known about the fine structure of chondroitin sulphate and dermatan sulphate in mast cells, little is understood about the composition of the heparin/heparan sulphate chains. Recent emphasis on the analysis of mast cell heparin from different species and tissues, arising from the use of this GAG in medicine, lead to the question of whether variations within heparin structures between mast cell populations are as significant as variations in the mix of chondroitins and heparins.
肥大细胞含有充满多种蛋白质的颗粒，这些蛋白质在脱颗粒时释放。蛋白聚糖丝甘蛋白带有一系列糖胺聚糖（GAG）侧链，有时是肝素，有时是硫酸软骨素或硫酸皮肤素。颗粒蛋白的紧密堆积依赖于携带这些GAG的丝甘蛋白的存在。肥大细胞的GAG在20世纪70年代和80年代得到了最深入的研究，尽管对肥大细胞中硫酸软骨素和硫酸皮肤素的精细结构有所了解，但对肝素/硫酸乙酰肝素链的组成却知之甚少。最近，由于这种GAG在医学上的应用，人们开始重视对不同物种和组织来源的肥大细胞肝素进行分析，这引发了一个问题，即肥大细胞群体之间肝素结构的差异是否与硫酸软骨素和肝素混合物的差异一样显著。
