新种拟沼泽小单孢菌，一种从拟沼泽金星蕨（Bak.）Ching根部分离得到的具有抗真菌活性的内生放线菌。
Micromonospora parathelypteridis sp. nov., an endophytic actinomycete with antifungal activity isolated from the root of Parathelypteris beddomei (Bak.) Ching.
作者信息
Zhao Shanshan, Liu Chongxi, Zheng Weiwei, Ma Zhaoxu, Cao Tingting, Zhao Junwei, Yan Kai, Xiang Wensheng, Wang Xiangjing
机构信息
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
出版信息
Int J Syst Evol Microbiol. 2017 Feb;67(2):268-274. doi: 10.1099/ijsem.0.001614. Epub 2017 Feb 21.
A novel endophytic actinomycete with antifungal activity, designated strain NEAU-JXY5T, was isolated from the root of Parathelypteris beddomei (Bak.) Ching. Strain NEAU-JXY5T showed closest 16S rRNA gene sequence similarity to Micromonospora luteifusca GUI2T (99.31 %), and phylogenetically clustered with Micromonospora noduli GUI43T (99.24 %), 'Micromonospora lycii' NEAU-gq11 (99.19 %), 'Micromonospora zeae' NEAU-gq9 (99.12 %), Micromonospora saelicesensis Lupac 09T (98.97 %), Micromonospora vinacea GUI63T (98.96 %), 'Micromonospora jinlongensis' NEAU-GRX11 (98.91 %), Micromonospora profundi DS3010T (98.77 %), Micromonospora zamorensis CR38T (98.76 %), Micromonospora chokoriensis 2-19(6)T (98.71 %), Micromonospora lupini Lupac 14NT (98.69 %), Micromonospora ureilytica GUI23T (98.69 %), Micromonospora violae NEAU-zh8T (98.57 %) and Micromonospora taraxaci NEAU-P5T (98.37 %). Phylogenetic analysis based on gyrB gene sequences also indicated that the isolate clustered with the above strains except M. violae NEAU-zh8T. A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that the strain could be readily distinguished from these closest phylogenetic relatives. Therefore, it is concluded that strain NEAU-JXY5T represents a novel species of the genus Micromonospora, for which the name Micromonospora parathelypteridis sp. nov. is proposed. The type strain is NEAU-JXY5T (=CGMCC 4.7347T=DSM 103125T).
从普通金星蕨（Parathelypteris beddomei (Bak.) Ching）的根部分离出了一株具有抗真菌活性的新型内生放线菌，命名为菌株NEAU-JXY5T。菌株NEAU-JXY5T的16S rRNA基因序列与淡黄小单孢菌（Micromonospora luteifusca）GUI2T的相似度最高（99.31%），在系统发育上与结节小单孢菌（Micromonospora noduli）GUI43T（99.24%）、“枸杞小单孢菌（Micromonospora lycii）”NEAU-gq11（99.19%）、“玉米小单孢菌（Micromonospora zeae）”NEAU-gq9（99.12%）、萨利塞斯小单孢菌（Micromonospora saelicesensis）Lupac 09T（98.97%）、酒红色小单孢菌（Micromonospora vinacea）GUI63T（98.96%）、“金龙小单孢菌（Micromonospora jinlongensis）”NEAU-GRX11（98.91%）、深海小单孢菌（Micromonospora profundi）DS3010T（98.77%）、萨莫拉小单孢菌（Micromonospora zamorensis）CR38T（98.76%）、乔科里小单孢菌（Micromonospora chokoriensis）2-19(6)T（98.71%）、羽扇豆小单孢菌（Micromonospora lupini）Lupac 14NT（98.69%）、解脲小单孢菌（Micromonospora ureilytica）GUI23T（98.69%）、堇菜小单孢菌（Micromonospora violae）NEAU-zh8T（98.57%）以及蒲公英小单孢菌（Micromonospora taraxaci）NEAU-P5T（98.37%）聚在一起。基于gyrB基因序列的系统发育分析也表明，该分离株与上述菌株聚在一起，但不包括堇菜小单孢菌NEAU-zh8T。DNA-DNA杂交结果和一些表型特征表明，该菌株可以很容易地与这些最近的系统发育亲缘种区分开来。因此，得出结论，菌株NEAU-JXY5T代表小单孢菌属的一个新物种，为此提出名为淡黄小单孢菌（Micromonospora parathelypteridis）的新种。模式菌株为NEAU-JXY5T（=CGMCC 4.7347T=DSM 103125T）。
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