Gao Ruixia, Liu Chongxi, Zhao Junwei, Jia Feiyu, Yu Chao, Yang Lingyu, Wang Xiangjing, Xiang Wensheng
School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
Antonie Van Leeuwenhoek. 2014 Feb;105(2):307-15. doi: 10.1007/s10482-013-0074-3. Epub 2013 Nov 21.
A novel actinomycete, designated strain NEAU-GRX11(T), was isolated from muddy soil collected from a stream of Jinlong Mountain in Harbin, north China. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-GRX11(T) showed highest similarity to Micromonospora zamorensis CR38(T) (99.2 %), Micromonospora saelicesensis Lupac 09(T) (99.0 %), Micromonospora chokoriensis 2-19/6(T) (98.7 %), Micromonospora coxensis 2-30-b/28(T) (98.5 %), Micromonospora aurantiaca ATCC 27029(T) (98.4 %) and Micromonospora lupini lupac 14N(T) (98.3 %). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-GRX11(T) was a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38(T), M. saelicesensis Lupac 09(T), M. chokoriensis 2-19/6(T) and M. lupini lupac 14N(T). A combination of DNA-DNA hybridization and some phenotypic characteristics indicated that the novel strain could be readily distinguished from these closest phylogenetic relatives. Therefore, it is proposed that NEAU-GRX11(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora jinlongensis sp. nov. is proposed. The type strain is NEAU-GRX11(T) (=CGMCC 4.7103(T)=DSM 45876(T)).
从中国北方哈尔滨金龙山溪流的泥质土壤中分离出一株新型放线菌，命名为菌株NEAU-GRX11(T)。该菌株具有小单孢菌属典型的形态和化学分类学特征。菌株NEAU-GRX11(T)的16S rRNA基因序列与扎莫罗小单孢菌CR38(T)（99.2%）、萨利塞斯小单孢菌Lupac 09(T)（99.0%）、乔科里小单孢菌2-19/6(T)（98.7%）、考克斯小单孢菌2-30-b/28(T)（98.5%）、橙黄小单孢菌ATCC 27029(T)（98.4%）和羽扇豆小单孢菌lupac 14N(T)（98.3%）的相似性最高。基于16S rRNA基因和gyrB基因的系统发育分析表明，菌株NEAU-GRX11(T)是小单孢菌属的成员，并支持其与扎莫罗小单孢菌CR38(T)、萨利塞斯小单孢菌Lupac 09(T)、乔科里小单孢菌2-19/6(T)和羽扇豆小单孢菌lupac 14N(T)的亲缘关系最近。DNA-DNA杂交和一些表型特征的组合表明，该新菌株可以很容易地与这些亲缘关系最近的菌株区分开来。因此，提议NEAU-GRX11(T)代表小单孢菌属的一个新物种，命名为金龙小单孢菌（Micromonospora jinlongensis sp. nov.）。模式菌株为NEAU-GRX11(T)（=CGMCC 4.7103(T)=DSM 45876(T)）。
