Meng Qingfeng, Zhao Yan, An LiXin, Li Xia, Liu Ping
Department of Ophthalmology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, China.
Department of Allergy, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, China.
Am J Transl Res. 2016 Nov 15;8(11):4968-4974. eCollection 2016.
Cinobufacini (Huachansu) is a Chinese medicine prepared from the skin of Cantor (Bufonidae), and has long been used in traditional Chinese medicine. In the present study, the anti-retinoblastoma constituent bufalin obtained from Cinobufacini was investigated. Treatment of human retinoblastoma (HXO-RB44) cells with bufalin induced apoptosis which was accompanied by a decrease in mitochondrial membrane potential, activation of caspase-9, caspase-8 and caspase-3, as well as changes in the expression of cytochrome C. Bufalin induced the cleavage of caspase-3 and apoptosis, and it was inhibited by both Z-LETD-FMK and Z-IETD-FMK treatment. Taken together, these results demonstrate that bufalin-induced apoptosis in human retinoblastoma (HXO-RB44) cells involved both intrinsic and extrinsic pathways.
华蟾素是一种由中华大蟾蜍（蟾蜍科）皮肤制备的中药，长期应用于传统中医。在本研究中，对从华蟾素中获得的抗视网膜母细胞瘤成分蟾毒灵进行了研究。用蟾毒灵处理人视网膜母细胞瘤（HXO-RB44）细胞可诱导细胞凋亡，同时伴有线粒体膜电位降低、半胱天冬酶-9、半胱天冬酶-8和半胱天冬酶-3激活以及细胞色素C表达变化。蟾毒灵诱导半胱天冬酶-3裂解和细胞凋亡，Z-LETD-FMK和Z-IETD-FMK处理均可抑制这种作用。综上所述，这些结果表明蟾毒灵诱导人视网膜母细胞瘤（HXO-RB44）细胞凋亡涉及内源性和外源性途径。
