Lanin A A, Fedotov I V, Ermakova Yu G, Sidorov-Biryukov D A, Fedotov A B, Hemmer P, Belousov V V, Zheltikov A M
Opt Lett. 2016 Dec 1;41(23):5563-5566. doi: 10.1364/OL.41.005563.
Optically detected electron spin resonance in fiber-coupled nitrogen-vacancy (NV) centers of diamond is used to demonstrate a fiber-optic quantum thermometry of individual thermogenetically activated neurons. Laser-induced temperature variations read out from single neurons with the NV-diamond fiber sensor are shown to strongly correlate with the fluorescence of calcium-ion sensors, serving as online indicators of the inward Ca current across the cell membrane of neurons expressing transient receptor potential (TRP) cation channels. Local laser heating above the TRP-channel activation threshold is shown to reproducibly evoke robust action potentials, visualized by calcium-ion-sensor-aided fluorescence imaging and detected as prominent characteristic waveforms in the time-resolved response of fluorescence Ca sensors.
利用光纤耦合金刚石中的氮空位（NV）中心的光学检测电子自旋共振，展示了对单个热激活神经元的光纤量子测温。通过NV金刚石光纤传感器从单个神经元读出的激光诱导温度变化，与钙离子传感器的荧光强烈相关，该荧光作为表达瞬时受体电位（TRP）阳离子通道的神经元细胞膜上内向钙电流的在线指标。高于TRP通道激活阈值的局部激光加热可重复引发强烈的动作电位，通过钙离子传感器辅助荧光成像可视化，并在荧光钙传感器的时间分辨响应中检测为突出的特征波形。
