Martins Rosany Lopes, Simões Ranggel Carvalho, Rabelo Érica de Menezes, Farias Ana Luzia Ferreira, Rodrigues Alex Bruno Lobato, Ramos Ryan da Silva, Fernandes João Batista, Santos Lourivaldo da Silva, de Almeida Sheylla Susan Moreira da Silva
Laboratory of Pharmacognosy and Phytochemistry-Federal University of Amapá, Macapá-Amapá, Brazil.
Department of Chemical, Federal University of São Carlos, São Carlos - São Paulo - Brazil.
PLoS One. 2016 Dec 1;11(12):e0166684. doi: 10.1371/journal.pone.0166684. eCollection 2016.
Aeollanthus suaveolens species popularly known as catinga de mulata belongs to the Lamiaceae family. In the Amazon region, it is used in folk medicine for the treatment of gastritis, convulsions of epileptic origin, stomach pain and diarrhea in the form of tea and juice. Essential oils have analgesic, anti-inflammatory, and antimicrobial activity. This study evaluated the chemical composition of the A. suaveolens essential oil, and its cytotoxic, antimicrobial and antioxidant activity on Artemia salina Leach. The plant species was collected in Fazendinha district in the city of Macapa-AP. The essential oil obtained from the process was performed by hydrodistillation and identification of components by gas chromatography coupled with mass spectrometer. The antioxidant activity was evaluated by the kidnapping method of 2,2- diphenyl -1-picrilhidrazil radical, while the cytotoxic activity was assessed using saline A. and the microbiological activity was carried out by microdilution method with Escherichia coli, Salmonella sp. and Staphylococcus aureus bacteria. In a chromatographic analysis, the major constituents found in the essential oil of A. suaveolens were (E) -β-farnesene (37.615%), Linalool (33.375%), α-Santalene (3.255%) and linalyl acetate (3.222%). The results showed that the Escherichia coli and Salmonella sp. bacteria were more susceptible to MIC 50 mg.mL-1 when compared with the Staphylococcus aureus bacterium MIC 100 mg.mL-1. With respect to MBC concentration of 100 mg.mL-1 it was sufficient to inhibit the growth of E. coli. The essential oil did not show antioxidant activity, however, has a high cytotoxic activity against the A. salina, LC50 8.90 μg.mL-1.
被普遍称为“卡廷加·德·穆拉塔”的香花藿香属于唇形科。在亚马逊地区，它以茶和果汁的形式被用于民间医学，治疗胃炎、癫痫性惊厥、胃痛和腹泻。精油具有镇痛、抗炎和抗菌活性。本研究评估了香花藿香精油的化学成分及其对卤虫的细胞毒性、抗菌和抗氧化活性。该植物物种采集于阿马帕州马卡帕市的法曾迪尼亚区。通过水蒸馏法从该植物中提取精油，并采用气相色谱-质谱联用仪鉴定其成分。抗氧化活性通过2,2-二苯基-1-苦基肼自由基捕获法进行评估，细胞毒性活性使用卤虫进行评估，微生物活性通过对大肠杆菌、沙门氏菌属和金黄色葡萄球菌采用微量稀释法进行测定。在色谱分析中，香花藿香精油中发现的主要成分是（E）-β-法尼烯（37.615%）、芳樟醇（33.375%）、α-檀香烯（3.255%）和乙酸芳樟酯（3.222%）。结果表明，与金黄色葡萄球菌的最低抑菌浓度（MIC）100 mg/mL相比，大肠杆菌和沙门氏菌属对MIC 50 mg/mL更敏感。就最低杀菌浓度（MBC）为100 mg/mL而言，足以抑制大肠杆菌的生长。该精油未表现出抗氧化活性，但对卤虫具有较高的细胞毒性活性，半数致死浓度（LC50）为8.90 μg/mL。
