Park Yu Jin, Lee Kyung-Ho, Kim Dong-Myung
Department of Chemical Engineering and Applied Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
Department of Chemical Engineering and Applied Chemistry, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
Anal Biochem. 2017 Feb 1;518:139-142. doi: 10.1016/j.ab.2016.11.019. Epub 2016 Nov 29.
We demonstrate the use of a cell-free protein synthesis system as a convenient tool for assessing the relative translational efficiencies of genes. When sfGFP was used as a common reporter gene and co-expressed with a series of target genes, the intensities of sfGFP fluorescence from the co-expression reactions were highly correlated with the individual expression levels of the co-expressed genes. The relative translational efficiencies of genes estimated by this method were reproducible when the same genes were expressed in transformed Escherichia coli, suggesting that this method could be used as a universal tool for prognostic assessment of translational efficiency.
我们展示了使用无细胞蛋白质合成系统作为评估基因相对翻译效率的便捷工具。当使用超折叠绿色荧光蛋白（sfGFP）作为通用报告基因并与一系列靶基因共表达时，共表达反应中sfGFP荧光强度与共表达基因的个体表达水平高度相关。当相同基因在转化的大肠杆菌中表达时，通过该方法估算的基因相对翻译效率具有可重复性，这表明该方法可作为翻译效率预后评估的通用工具。
