Kucheryavykh Lilia Y, Dávila-Rodríguez Josué, Rivera-Aponte David E, Zueva Lidia V, Washington A Valance, Sanabria Priscilla, Inyushin Mikhail Y
Department of Biochemistry, School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA.
School of Medicine, Universidad Central del Caribe, Bayamon, PR 00960-6032, (P.O. Box 60327), USA.
Brain Res Bull. 2017 Jan;128:98-105. doi: 10.1016/j.brainresbull.2016.11.008. Epub 2016 Nov 28.
Platelets contain beta-amyloid precursor protein (APP) as well as Aβ peptide (Aβ) that can be released upon activation. During thrombosis, platelets are concentrated in clots and activated.
We used in vivo fluorescent analysis and electron microscopy in mice to determine to what degree platelets are concentrated in clots. We used immunostaining to visualize Aβ after photothrombosis in mouse brains.
Both in vivo results and electron microscopy revealed that platelets were 300-500 times more concentrated in clots than in non-clotted blood. After thrombosis in control mice, but not in thrombocytopenic animals, Aβ immunofluorescence was present inside blood vessels in the visual cortex and around capillaries in the entorhinal cortex.
The increased concentration of platelets allows enhanced release of Aβ during thrombosis, suggesting an additional source of Aβ in the brains of Alzheimer's patients that may arise if frequent micro-thrombosis events occur in their brains.
血小板含有β-淀粉样前体蛋白（APP）以及激活时可释放的Aβ肽（Aβ）。在血栓形成过程中，血小板聚集在凝块中并被激活。
我们在小鼠体内进行荧光分析和电子显微镜检查，以确定血小板在凝块中的聚集程度。我们使用免疫染色来观察小鼠大脑光血栓形成后的Aβ。
体内结果和电子显微镜检查均显示，血小板在凝块中的浓度比在非凝血血液中高300 - 500倍。在对照小鼠血栓形成后，而非血小板减少动物中，视觉皮层的血管内和内嗅皮层的毛细血管周围存在Aβ免疫荧光。
血小板浓度的增加使得血栓形成过程中Aβ的释放增强，这表明如果阿尔茨海默病患者大脑中频繁发生微血栓事件，可能会在其大脑中产生Aβ的额外来源。
