源自胚胎干细胞和诱导多能干细胞的心肌细胞中的基因转移
Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells.
作者信息
Stillitano Francesca, Karakikes Ioannis, Hajjar Roger J
机构信息
Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1030, New York, NY, 10029, USA.
Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, USA.
出版信息
Methods Mol Biol. 2017;1521:183-193. doi: 10.1007/978-1-4939-6588-5_12.
The advent of human induced pluripotent stem cell (hiPSC) technology has produced patient-specific hiPSC derived cardiomyocytes (hiPSC-CMs) that can be used as a platform to study cardiac diseases and to explore new therapies.The ability to genetically manipulate hiPSC-CMs not only is essential for identifying the structural and/or functional role of a protein but can also provide valuable information regarding therapeutic applications. In this chapter, we describe protocols for culture, maintenance, and cardiac differentiation of hiPSCs. Then, we provide a basic procedure to transduce hiPSC-CMs.
人类诱导多能干细胞（hiPSC）技术的出现产生了患者特异性的hiPSC衍生心肌细胞（hiPSC-CMs），其可作为研究心脏疾病和探索新疗法的平台。对hiPSC-CMs进行基因操作的能力不仅对于确定蛋白质的结构和/或功能作用至关重要，还可为治疗应用提供有价值的信息。在本章中，我们描述了hiPSC的培养、维持和心脏分化方案。然后，我们提供了转导hiPSC-CMs的基本程序。
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