Numanović Fatima, Smajlović Jasmina, Gegić Merima, Delibegović Zineta, Bektaš Sabaheta, Halilović Emir, Nurkić Jasmina
Institute of Microbiology, Polyclinic for Laboratory Diagnostics, University Clinical Center Tuzla, Tuzla, Bosnia and Herzegovina.
Department of Microbiology, Institute for Public Health of Canton Sarajevo, Sarajevo; Bosnia and Herzegovina.
Med Glas (Zenica). 2017 Feb 1;14(1):98-105. doi: 10.17392/876-16.
Aim To determine the prevalence rate and resistance profile of Streptococcus agalactiae (S. agalactiae) in vaginal swabs of pregnant and adult non-pregnant women in the Tuzla region, Bosnia and Herzegovina (B&H), as well as its association with other aerobic bacteria. Methods This prospective study included 200 women, 100 pregnant and 100 adult non-pregnant. The research was conducted at the Institute of Microbiology, University Clinical Center Tuzla from October to December 2015. Standard aerobic microbiological techniques were used for isolation and identification of S. agalactiae and other aerobic bacteria. Antimicrobial susceptibility was determined by the disk diffusion and microdilution method(VITEK 2/AES instrument). Results Among 200 vaginal swabs, 17 (8.50%) were positive for S. agalactiae, e. g., 7% (7/100) of pregnant and 10% (10/100) of adult non-pregnant women. In the pregnant group, 71.4% (5/7) of S. agalactiae isolates were susceptible to clindamycin and 85.7%(6/7) to erythromycin. In the adult non-pregnant group, only resistance to clindamycin was observed in one patient (1/10; 10%). S. agalactiae as single pathogen was isolated in 57.14% (4/7) of pregnant and 60% (6/10) of adult non-pregnant S. agalactiae positive women. In mixed microbial cultures S. agalactiae was most frequently associated with Enterococcus faecalis and Escherichia coli. Conclusion The rate of S. agalactiae positive women in the population of pregnant and adult non-pregnant women of Tuzla Canton, B&H is comparable with other European countries. Large studies are needed to develop a common national strategy for the prevention of S. agalactiae infection in B&H, especially during pregnancy.
目的 确定波斯尼亚和黑塞哥维那（波黑）图兹拉地区孕妇及成年非孕妇阴道拭子中无乳链球菌（S. agalactiae）的流行率和耐药情况，以及其与其他需氧菌的关联。方法 这项前瞻性研究纳入了200名女性，其中100名孕妇和100名成年非孕妇。研究于2015年10月至12月在图兹拉大学临床中心微生物研究所进行。采用标准需氧微生物技术分离和鉴定无乳链球菌及其他需氧菌。通过纸片扩散法和微量稀释法（VITEK 2/AES仪器）测定抗菌药物敏感性。结果 在200份阴道拭子中，17份（8.50%）无乳链球菌呈阳性，即孕妇中为7%（7/100），成年非孕妇中为10%（10/100）。在孕妇组中，71.4%（5/7）的无乳链球菌分离株对克林霉素敏感，85.7%（6/7）对红霉素敏感。在成年非孕妇组中，仅1例患者（1/10；10%）对克林霉素耐药。在57.14%（4/7）的孕妇和60%（6/10）的成年非孕妇无乳链球菌阳性女性中，无乳链球菌作为单一病原体被分离出来。在混合微生物培养中，无乳链球菌最常与粪肠球菌和大肠杆菌相关联。结论 波黑图兹拉州孕妇及成年非孕妇人群中无乳链球菌阳性女性的比例与其他欧洲国家相当。需要开展大型研究以制定波黑预防无乳链球菌感染的国家通用策略，尤其是在孕期。
