Li Chang-Chun, Wang Yong, Li Guo-Yuan, Yun Yue-Li, Dai Yu-Jun, Chen Jian, Peng Yu
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China.
Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, China.
Int J Mol Sci. 2016 Dec 3;17(12):2033. doi: 10.3390/ijms17122033.
is one of the most common wandering spiders in agricultural fields and a potentially good bioindicator for heavy metal contamination. However, little is known about the mechanisms by which spiders respond to heavy metals at the molecular level. In the present study, high-throughput transcriptome sequencing was employed to characterize the de novo transcriptome of the spiders and to identify differentially expressed genes (DEGs) after cadmium exposure. We obtained 60,489 assembled unigenes, 18,773 of which were annotated in the public databases. A total of 2939 and 2491 DEGs were detected between the libraries of two Cd-treated groups and the control. Functional enrichment analysis revealed that metabolism processes and digestive system function were predominately enriched in response to Cd stress. At the cellular and molecular levels, significantly enriched pathways in lysosomes and phagosomes as well as replication, recombination and repair demonstrated that oxidative damage resulted from Cd exposure. Based on the selected DEGs, certain critical genes involved in defence and detoxification were analysed. These results may elucidate the molecular mechanisms underlying spiders' responses to heavy metal stress.
是农田中最常见的游走蜘蛛之一，也是重金属污染潜在的良好生物指示物种。然而，关于蜘蛛在分子水平上对重金属作出反应的机制，人们了解甚少。在本研究中，采用高通量转录组测序来表征蜘蛛的从头转录组，并鉴定镉暴露后的差异表达基因（DEG）。我们获得了60489个组装的单基因，其中18773个在公共数据库中得到注释。在两个镉处理组和对照组的文库之间共检测到2939个和2491个DEG。功能富集分析表明，代谢过程和消化系统功能在对镉胁迫的反应中占主导地位。在细胞和分子水平上，溶酶体和吞噬体以及复制、重组和修复中显著富集的途径表明，镉暴露导致了氧化损伤。基于选定的DEG，分析了参与防御和解毒的某些关键基因。这些结果可能阐明蜘蛛对重金属胁迫反应的分子机制。
