Studies of ventromedial hypothalamic afferents in the rat using three methods of HRP application.

The afferent neural connections of the ventromedial nucleus of the rat hypothalamus (VMH) have been studied in detail using three horseradish peroxidase (HRP) application methods: HRP crystal implants, HRP-gel implants, and iontophoretic deposition of the enzyme. Examination of the cases in which the retrograde tracer was best confined to various subdivisions of the nucleus revealed that the septal area projects only to the ventrolateral VMH, and that the medial preoptic area, rostral lateral hypothalamus, and the ventral subiculum project mainly to the ventrolateral VMH. Thus, the subdivision of the VMH that contains the highest density of estradiol-concentrating neurons (Morrell et al. 1986) receives a larger set of inputs than the rostral and central parts of the nucleus. The central subdivision receives a more restricted set of projections than either the medial or the lateral regions. These studies suggest that there may be partial anatomical segregation of neural inputs to the various subdivisions of the VMH.