Scharf M, Engels J, Tripier D
Institut für organische Chemie, Johann Wolfgang v. Goethe Universität, Frankfurt, FRG.
FEBS Lett. 1989 Sep 11;255(1):105-10. doi: 10.1016/0014-5793(89)81070-1.
Crude hirudin (12.7 U/micrograms), a complex mixture of polypeptides obtained from the leech, could be separated by microbore HPLC. A combination of amino acid analysis, N-terminal microsequencing and chemical as well as enzymatic fragmentation made the primary sequence of the new isohirudins Ia-IIIb' accessible. The biological activity determined in the thrombin inhibition test showed a comparable value for all of these compounds. The results presented address the question as to whether these isohirudins are true mutations from a family of genes or a family of leeches.
粗制水蛭素（12.7单位/微克）是从水蛭中获得的一种多肽混合物，可通过微径高效液相色谱法进行分离。氨基酸分析、N端微量测序以及化学和酶促裂解相结合，使得新异水蛭素Ia - IIIb'的一级序列得以确定。凝血酶抑制试验中测定的生物活性表明，所有这些化合物的值相当。所呈现的结果解决了这些异水蛭素是来自一个基因家族的真正突变体还是一个水蛭家族的问题。
