通过文库筛选鉴定微小RNA的小分子调节剂
Identification of Small Molecule Modulators of MicroRNA by Library Screening.
作者信息
Xiao Zhangang, Chen Yangchao
机构信息
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
State Key Laboratory of Digestive Disease, Institute of Digestive Disease, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
出版信息
Methods Mol Biol. 2017;1517:169-178. doi: 10.1007/978-1-4939-6563-2_12.
MicroRNAs (miRNAs) function as oncogenes or tumor suppressors and are dysregulated in cancer. miRNAs therefore represent promising therapeutic targets for cancer. Small molecules that could modulate the expression of miRNAs would thus have potential as anticancer agents. Library screening of small molecules targeting miRNAs is a useful technology platform for anticancer drug development. Here, we describe a hepatocellular carcinoma (HCC) cell-based luciferase reporter system which could be used to screen for small molecule modulators of tumor suppressor microRNA-34a.
微小RNA（miRNA）发挥癌基因或肿瘤抑制因子的作用，且在癌症中表达失调。因此，miRNA是很有前景的癌症治疗靶点。能够调节miRNA表达的小分子因而具有作为抗癌药物的潜力。针对miRNA的小分子文库筛选是抗癌药物研发的一个有用技术平台。在此，我们描述了一种基于肝癌（HCC）细胞的荧光素酶报告系统，该系统可用于筛选肿瘤抑制性微小RNA-34a的小分子调节剂。
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