服用辅助性他莫昔芬的巴基斯坦乳腺癌患者中CYP2D6*10基因型的频率。

Frequency of CYP2D6*10 genotypes in Pakistani breast cancer patients taking adjuvant tamoxifen.

作者信息

Nazir Nusrat, Waheed Akbar, Farhat Kulsoom, Ismail Muhammad, Mansoor Qaisar

机构信息

Department of Pharmacology & Therapeutics Army Medical College (National University of Sciences and Technology), Islamabad.

Institute of Biomedical and Genetic Engineering, Islamabad.

出版信息

J Pak Med Assoc. 2016 Dec;66(12):1554-1558.

PMID:27924964

Abstract

OBJECTIVE

To investigate the frequency of cytochrome P2D6*10 in breast cancer patients.

METHODS

This retrospective study was conducted at the Nuclear Medicine, Oncology and Radiotherapy Institute, Islamabad, and the Combined Military Hospital, Rawalpindi, Pakistan, and comprised medical records of breast cancer patients from January 2000 to September 2013. Pre- and post-menopausal women with diagnosed breast cancer who were advised 20mg/day of tamoxifen as adjuvant therapy were included. The frequency of the cytochrome was determined using polymerase chain reaction-restriction fragment length polymorphism analysis.

RESULTS

Of the 232 participants, 25(10.8%) had stage I disease, 127(54.7%) had stage II and 80(34.5%) had stage III disease. The overall mean age was 46.9±9.9 years. The allele frequency of cytochrome CYP2D61 was 431(93%) and that of CYP2D610 was 33(7 %). Pakistanis differed significantly from the Asian populations and other ethnic groups in the distribution of the allele cytochrome, but its frequency was comparable to South Indians.

CONCLUSIONS

The frequency of CYP2D6*10 allele in Pakistani breast cancer women was comparable to the South Indians, but was significantly lower than other populations.

摘要

目的

调查细胞色素P2D6*10在乳腺癌患者中的出现频率。

方法

这项回顾性研究在巴基斯坦伊斯兰堡的核医学、肿瘤学和放射治疗研究所及拉瓦尔品第的联合军事医院开展，纳入了2000年1月至2013年9月乳腺癌患者的医疗记录。纳入了诊断为乳腺癌且被建议每日服用20mg他莫昔芬作为辅助治疗的绝经前和绝经后女性。使用聚合酶链反应-限制性片段长度多态性分析来确定细胞色素的出现频率。

结果

232名参与者中，25名（10.8%）患有I期疾病，127名（54.7%）患有II期疾病，80名（34.5%）患有III期疾病。总体平均年龄为46.9±9.9岁。细胞色素CYP2D61的等位基因频率为431（93%），CYP2D610的等位基因频率为33（7%）。巴基斯坦人与亚洲人群及其他种族群体在细胞色素等位基因分布上存在显著差异，但其频率与南印度人相当。