石蜡包埋的早期子宫内膜癌DNA指数和S期的流式细胞术测量：一项重要的预后指标。

Flow cytometric measurements of DNA index and S-phase on paraffin-embedded early stage endometrial cancer: an important prognostic indicator.

作者信息

Rosenberg P, Wingren S, Simonsen E, Stàl O, Risberg B, Nordenskjöld B

机构信息

Department of Gynecological Oncology, University Hospital, Linköping, Sweden.

出版信息

Gynecol Oncol. 1989 Oct;35(1):50-4. doi: 10.1016/0090-8258(89)90010-3.

DOI:10.1016/0090-8258(89)90010-3

PMID:2792902

Abstract

Paraffin-embedded curettage material from 120 patients with stage I and II endometrial cancer were analyzed by flow cytometry. The follow-up time in all cases was at least 5 years or until death. It was possible to determine DNA ploidy in 111 cases and S-phase was also evaluated in 92/111 cases. DNA ploidy and S-phase were then compared with the FIGO grading and clinical outcome. DNA ploidy results showed that 11% of grade 1 tumors were aneuploid, 14% of grade 2, 42% of grade 3, and 85% of the cases of uterine papillary serous carcinoma (UPSC) were aneuploid. Patients with tumors that showed an S-phase fraction below 5% had a cumulative 5-year cancer mortality of 7% whereas 49% of the patients with tumors having S-phase fraction above 10% died of their cancer. The prognostic significance of DNA and S-phase correlated fairly well with the FIGO grading as determined by a pathologist with special interest in gynecologic oncology and the DNA parameters added prognostic information independent of the FIGO grading.

摘要

对120例I期和II期子宫内膜癌患者的石蜡包埋刮宫材料进行了流式细胞术分析。所有病例的随访时间至少为5年或直至死亡。111例病例可确定DNA倍体，92/111例病例还评估了S期。然后将DNA倍体和S期与国际妇产科联盟（FIGO）分级及临床结果进行比较。DNA倍体结果显示，1级肿瘤中有11%为非整倍体，2级为14%，3级为42%，子宫乳头状浆液性癌（UPSC）病例中有85%为非整倍体。S期分数低于5%的肿瘤患者5年累积癌症死亡率为7%，而S期分数高于10%的肿瘤患者中有49%死于癌症。DNA和S期的预后意义与FIGO分级有较好的相关性，由一位对妇科肿瘤学有特殊兴趣的病理学家确定，并且DNA参数提供了独立于FIGO分级的预后信息。