Pauchet Yannick, Bretschneider Anne, Augustin Sylvie, Heckel David G
Department of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, Jena 07745, Germany.
Unité de Zoologie Forestière, Institut National de la Recherche Agronomique (INRA), 2163 Avenue de la Pomme de Pin, CS 40001 Ardon, Orléans 45075 CEDEX 2, France.
Toxins (Basel). 2016 Dec 5;8(12):362. doi: 10.3390/toxins8120362.
is a polyvoltine oligophagous leaf beetle responsible for massive attacks on poplar trees. This beetle is an important model for understanding mechanisms of resistance to (Bt) insecticidal toxins, because a resistant strain has been found that can survive and reproduce on transgenic poplar trees expressing high levels of the Cry3Aa Bt toxin. Resistance to Cry3Aa in this strain is recessive and is controlled by a single autosomal locus. We used a larval midgut transcriptome for to search for candidate resistance genes. We discovered a mutation in an ABC protein, member of the B subfamily homologous to P-glycoprotein, which is genetically linked to Cry3Aa resistance in . Cultured insect cells heterologously expressing this ABC protein swell and lyse when incubated with Cry3Aa toxin. In light of previous findings in Lepidoptera implicating A subfamily ABC proteins as receptors for Cry2A toxins and C subfamily proteins as receptors for Cry1A and Cry1C toxins, this result suggests that ABC proteins may be targets of insecticidal three-domain Bt toxins in Coleoptera as well.
是一种多食性寡食性叶甲虫，对杨树造成大规模侵害。这种甲虫是理解对（Bt）杀虫毒素抗性机制的重要模型，因为已发现一个抗性品系能够在表达高水平Cry3Aa Bt毒素的转基因杨树上存活和繁殖。该品系对Cry3Aa的抗性是隐性的，由一个常染色体位点控制。我们利用的幼虫中肠转录组来寻找候选抗性基因。我们在一种与P-糖蛋白同源的B亚家族ABC蛋白中发现了一个突变，该突变在基因上与的Cry3Aa抗性相关。当与Cry3Aa毒素一起孵育时，异源表达这种ABC蛋白的培养昆虫细胞会肿胀并裂解。鉴于之前在鳞翅目中的发现表明A亚家族ABC蛋白是Cry2A毒素的受体，C亚家族蛋白是Cry1A和Cry1C毒素的受体，这一结果表明ABC蛋白也可能是鞘翅目昆虫中杀虫三结构域Bt毒素的作用靶点。
