Gupta P K, Appannanavar S B, Mohan B, Taneja N
Department of Microbiology, Dr. RPGMC, Kangra, Himachal Pradesh, India.
Department of Microbiology, SDM Medical College, Dharwad, Karnataka, India.
Indian J Med Microbiol. 2016 Oct-Dec;34(4):526-528. doi: 10.4103/0255-0857.195355.
Laboratory diagnosis of shigellosis using conventional culture technique is limited by lower sensitivity and higher turnaround time. Here, we have evaluated the role of polymerase chain reaction from stool samples after enrichment in Escherichia coli medium for detection of Shigellae. The technique not only increased the sensitivity but also decreased the turnaround time.
使用传统培养技术对志贺菌病进行实验室诊断受到较低敏感性和较长周转时间的限制。在此，我们评估了在大肠杆菌培养基中富集后从粪便样本进行聚合酶链反应在检测志贺菌方面的作用。该技术不仅提高了敏感性，还缩短了周转时间。
