Krüger-Genge A, Fuhrmann R, Jung F, Franke R P
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Abteilung Biomaterialien, Zentralinstitut für Biomedizinische Technik, Universität Ulm, Ulm, Germany.
Clin Hemorheol Microcirc. 2016;64(4):867-874. doi: 10.3233/CH-168051.
The endothelialization of cardiovascular prostheses is known to improve their haemocompatibility. As such body-foreign materials often do not endothelialize spontaneously. A lot of in vitro studies are ongoing how endothelialization of biomaterials can be improved. In this study the influence of different components of a tissue-typical extracellular matrix (ECM) like laminin, fibronectin or gelatin on the formation of an endothelial cell monolayer and on the shear resistance of adherent cells on these substrates was studied.The study revealed that the density of human venous endothelial cells (HUVEC) monolayers differed markedly between cells grown on a natural ECM and cells grown on singularized components of an ECM (p < 0.001). Only HUVEC grown on laminin showed similar densities and a stress fiber pattern comparable to HUVEC grown on the ECM. HUVEC grown on gelatin- or fibronectin-coated coverslips were less firmly attached to the substrate; frequently individual HUVEC and even groups of cells detached.Concluding it seems that coating of implants with laminin supports the formation of shear resistant endothelial cell (EC) monolayer - superior to other ECM components.
已知心血管假体的内皮化可改善其血液相容性。然而，这类异体材料通常不会自发地实现内皮化。目前正在进行大量关于如何改善生物材料内皮化的体外研究。在本研究中，研究了组织典型细胞外基质（ECM）的不同成分，如层粘连蛋白、纤连蛋白或明胶，对内皮细胞单层形成以及这些底物上贴壁细胞抗剪切力的影响。研究表明，在天然ECM上生长的细胞与在ECM单一成分上生长的细胞相比，人静脉内皮细胞（HUVEC）单层的密度存在显著差异（p<0.001）。只有在层粘连蛋白上生长的HUVEC显示出与在ECM上生长的HUVEC相似的密度和应力纤维模式。在明胶或纤连蛋白包被的盖玻片上生长的HUVEC与底物的附着不那么牢固；单个HUVEC甚至细胞群经常脱落。结论是，用层粘连蛋白包被植入物似乎有助于形成抗剪切的内皮细胞（EC）单层——优于其他ECM成分。
