Labriola Nicholas R, Mathiowitz Edith, Darling Eric M
Center for Biomedical Engineering, Brown University, Providence, RI 02912, USA.
Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI 02912, USA.
Biomater Sci. 2016 Dec 20;5(1):41-45. doi: 10.1039/c6bm00692b.
Inverse emulsification was used to fabricate polyacrylamide (PAAm) microbeads with size and elastic properties similar to typical, mammalian cells. These biomimicking microbeads could be fluorescently stained and functionalized with a collagen type-I coating, post-polymerization, for tracking bead locations and promoting cell recognition/binding, respectively. By occupying a previously unfilled range of sizes and mechanical properties, these microbeads may find unique use in both biomedical and materials applications.
采用反相乳化法制备了尺寸和弹性性能与典型哺乳动物细胞相似的聚丙烯酰胺(PAAm)微珠。这些仿生微珠可以进行荧光染色,并在聚合后用I型胶原蛋白涂层进行功能化处理,分别用于追踪微珠位置和促进细胞识别/结合。通过占据以前未被填充的尺寸和机械性能范围,这些微珠可能在生物医学和材料应用中找到独特的用途。