Yao Chunhe, Carlisi Cristina, Li Yuning, Chen Da, Ding Jianfu, Feng Yong-Lai
Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, Environmental and Radiation Health Sciences Directorate, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, Ontario, Canada.
Department of Chemical Engineering, Department of Chemistry, and Waterloo Institute for Nanotechnology (WIN), University of Waterloo, Waterloo, Ontario, Canada.
PLoS One. 2016 Dec 9;11(12):e0167796. doi: 10.1371/journal.pone.0167796. eCollection 2016.
Increasing use of single-walled carbon nanotubes (SWCNTs) necessitates a novel method for hazard risk assessment. In this work, we investigated the interaction of several types of commercial SWCNTs with single-stranded (ss) and double-stranded (ds) DNA oligonucleotides (20-mer and 20 bp). Based on the results achieved, we proposed a novel assay that employed the DNA interaction potency to assess the hazard risk of SWCNTs. It was found that SWCNTs in different sizes or different batches of the same product number of SWCNTs showed dramatically different potency of interaction with DNAs. In addition, the same SWCNTs also exerted strikingly different interaction potency with ss- versus ds- DNAs. The interaction rates of SWCNTs with DNAs were investigated, which could be utilized as the indicator of potential hazard for acute exposure. Compared to solid SWCNTs, the SWCNTs dispersed in liquid medium (2% sodium cholate solution) exhibited dramatically different interaction potency with DNAs. This indicates that the exposure medium may greatly influence the subsequent toxicity and hazard risk produced by SWCNTs. Based on the findings of dose-dependences and time-dependences from the interactions between SWCNTs and DNAs, a new chemistry based assay for hazard risk assessment of nanomaterials including SWCNTs has been presented.
单壁碳纳米管（SWCNTs）使用的增加使得有必要采用一种新的危害风险评估方法。在这项工作中，我们研究了几种类型的商业单壁碳纳米管与单链（ss）和双链（ds）DNA寡核苷酸（20聚体和20碱基对）之间的相互作用。基于所取得的结果，我们提出了一种新的检测方法，该方法利用DNA相互作用能力来评估单壁碳纳米管的危害风险。研究发现，不同尺寸或同一产品编号的不同批次的单壁碳纳米管与DNA的相互作用能力存在显著差异。此外，相同的单壁碳纳米管与单链DNA和双链DNA的相互作用能力也存在显著差异。我们研究了单壁碳纳米管与DNA的相互作用速率，该速率可作为急性暴露潜在危害的指标。与固态单壁碳纳米管相比，分散在液体介质（2%胆酸钠溶液）中的单壁碳纳米管与DNA的相互作用能力存在显著差异。这表明暴露介质可能会极大地影响单壁碳纳米管产生的后续毒性和危害风险。基于单壁碳纳米管与DNA相互作用的剂量依赖性和时间依赖性研究结果，我们提出了一种基于化学的新检测方法，用于评估包括单壁碳纳米管在内的纳米材料的危害风险。
