Sun Cuixia, Dai Lei, Gao Yanxiang
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University , Beijing 100083, China.
Biomacromolecules. 2016 Dec 12;17(12):3973-3985. doi: 10.1021/acs.biomac.6b01362. Epub 2016 Nov 30.
Propylene glycol alginate (PGA) was found to be able to dissolve in aqueous ethanol solution and applied to interact with zein to form a noncovalent binary complex by the antisolvent coprecipitation method at pH 4.0. Quercetagetin (Q) was employed to explore the Q-delivery potential of Zein-PGA binary complex. A fruit tree-like microstructure was observed for Zein-PGA binary complex as its "branches" were closely adsorbed by zein particles. A solid sponge-like entity was formed after lyophilization of Zein-PGA binary complex colloidal dispersion. A synergistic effect was found between zein and PGA on improving the entrapment efficiency and loading capacity of Q. The incorporation of Q at a high concentration induced a significant effect on the tertiary structure of zein. Electrostatic attraction, hydrogen bond, and hydrophobic effects were mainly involved in the interactions between zein and PGA. Schematics with four possible structures were proposed to explain the formation mechanism of composites.
研究发现，海藻酸丙二醇酯（PGA）能够溶解于乙醇水溶液中，并通过反溶剂共沉淀法在pH 4.0条件下与玉米醇溶蛋白相互作用，形成非共价二元复合物。采用槲皮万寿菊素（Q）来探究玉米醇溶蛋白-海藻酸丙二醇酯二元复合物的Q递送潜力。观察到玉米醇溶蛋白-海藻酸丙二醇酯二元复合物呈现出类似果树的微观结构，其“树枝”被玉米醇溶蛋白颗粒紧密吸附。玉米醇溶蛋白-海藻酸丙二醇酯二元复合物胶体分散液冻干后形成了固体海绵状实体。发现玉米醇溶蛋白和海藻酸丙二醇酯在提高槲皮万寿菊素的包封率和载药量方面具有协同作用。高浓度槲皮万寿菊素的掺入对玉米醇溶蛋白的三级结构产生了显著影响。静电吸引、氢键和疏水作用主要参与了玉米醇溶蛋白与海藻酸丙二醇酯之间的相互作用。提出了四种可能结构的示意图来解释复合材料的形成机制。
