Bertheussen K, Holst N, Forsdahl F, Høie K E
Institute of Medical Biology, University of Tromsø, Norway.
Hum Reprod. 1989 Jul;4(5):531-5. doi: 10.1093/oxfordjournals.humrep.a136939.
A new, simple and sensitive bioassay for quality testing in IVF is described. This bioassay (Medi-Cult Hybritest, GEA Ltd, Biotech Division, Hvidovre, Denmark) is based on the culture of the rapidly growing mouse hybridoma cell line 1E6 in a defined serum-free culture medium. The use of serum-free conditions greatly increases the sensitivity to toxic substances, due to the absence of binding proteins. The testing of known toxic agents showed that this assay disclosed cytotoxicity with a high sensitivity. The Hybritest thus provides a simple yet sensitive and reproducible bioassay for quality control of culture media, water, chemical compounds and equipment in an IVF programme. Tests of different batches of culture media showed that media for IVF should be processed from powder and high-quality sterile water. It is important not only to test the single components of the culture media, but also the final product in a sensitive test system.
本文描述了一种用于体外受精（IVF）质量检测的新型、简单且灵敏的生物测定法。这种生物测定法（Medi-Cult Hybritest，丹麦海勒鲁普市GEA有限公司生物技术部）基于快速生长的小鼠杂交瘤细胞系1E6在特定无血清培养基中的培养。由于不存在结合蛋白，无血清条件的使用大大提高了对有毒物质的敏感性。对已知有毒试剂的测试表明，该测定法能高度灵敏地检测出细胞毒性。因此，Hybritest为体外受精程序中培养基、水、化合物和设备的质量控制提供了一种简单、灵敏且可重复的生物测定法。对不同批次培养基的测试表明，体外受精用培养基应由粉末和高质量无菌水配制而成。不仅要在灵敏的测试系统中检测培养基的单一成分，还要检测最终产品，这一点很重要。
