Dias Disney Ribeiro, Silva Monique Suela, Cristina de Souza Angélica, Magalhăes-Guedes Karina Teixeira, Ribeiro Fernanda Severo de Rezende, Schwan Rosane Freitas
Department of Food Science, Federal University of Lavras (UFLA), University Campus, 37200-000 Lavras, MG, Brazil.
Department of Biology, Federal University of Lavras (UFLA), University Campus, 37200-000 Lavras, MG, Brazil.
Food Technol Biotechnol. 2016 Sep;54(3):351-359. doi: 10.17113/ftb.54.03.16.4416.
Cell immobilization comprises the retention of metabolically active cells inside a polymeric matrix. In this study, the production of jabuticaba () vinegar using immobilized and cells is proposed as a new method to prevent losses of jabuticaba fruit surplus. The pulp of jabuticaba was processed and CCMA 0200 was used to ferment the must for jabuticaba wine production. Sugars, alcohols (ethanol and glycerol) and organic acids were assayed by high-performance liquid chromatography. Volatile compounds were determined by gas chromatography-flame ionization detector. The ethanol content of the produced jabuticaba wine was approx. 74.8 g/L (9.5% by volume) after 168 h of fermentation. Acetic acid fermentation for vinegar production was performed using a mixed culture of immobilized CCT 0190 and CCMA 0350 cells. The acetic acid yield was 74.4% and productivity was 0.29 g/(L·h). The vinegar had particularly high concentrations of citric (6.67 g/L), malic (7.02 g/L) and succinic (5.60 g/L) acids. These organic acids give a suitable taste and flavour to the vinegar. Seventeen compounds (aldehydes, higher alcohols, terpene, acetate, diether, furans, acids, ketones and ethyl esters) were identified in the jabuticaba vinegar. In conclusion, vinegar was successfully produced from jabuticaba fruits using yeast and immobilized mixed cultures of and . To the best of our knowledge, this is the first study to use mixed culture of immobilized cells for the production of jabuticaba vinegar.
细胞固定化包括将代谢活性细胞保留在聚合物基质内。在本研究中,提出使用固定化的[具体菌种1]和[具体菌种2]细胞生产巴西葡萄醋,作为防止巴西葡萄果实过剩损失的一种新方法。对巴西葡萄的果肉进行加工,并使用CCMA 0200发酵葡萄汁以生产巴西葡萄酒。通过高效液相色谱法测定糖、醇(乙醇和甘油)和有机酸。通过气相色谱 - 火焰离子化检测器测定挥发性化合物。发酵168小时后,所生产的巴西葡萄酒的乙醇含量约为74.8 g/L(体积分数为9.5%)。使用固定化的CCT 0190和CCMA 0350细胞的混合培养物进行醋酸发酵以生产醋。醋酸产率为74.4%,生产率为0.29 g/(L·h)。该醋中柠檬酸(6.67 g/L)、苹果酸(7.02 g/L)和琥珀酸(5.60 g/L)的浓度特别高。这些有机酸赋予了醋适宜的口感和风味。在巴西葡萄醋中鉴定出了17种化合物(醛、高级醇、萜烯、乙酸酯、二醚、呋喃、酸、酮和乙酯)。总之,使用酵母和固定化的[具体菌种1]与[具体菌种2]混合培养物成功地从巴西葡萄果实中生产出了醋。据我们所知,这是首次使用固定化细胞混合培养物生产巴西葡萄醋的研究。