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人类结肠腺癌中表达序列标签的生物信息学分析与验证

Bioinformatics Analysis and Validation of the Expressed Sequences Tag in Human Colorectal Adenocarcinoma.

作者信息

Chen Yao, Zhang Chun Le, Shen Yong Qiang, Wang Li Cheng

机构信息

Department of Anatomy, Basic Medical and Legal Medical Institute of Sichuan university. Chengdu, Sichuan, China. 610041.

出版信息

Gastroenterology Res. 2009 Apr;2(2):110-114. doi: 10.4021/gr2009.04.1287. Epub 2009 Mar 20.

DOI:10.4021/gr2009.04.1287
PMID:27956963
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5139827/
Abstract

BACKGROUND

This study was to investigate some new pathological genes in colorectal adenocarcinoma of human.

METHODS

Human colorectal adenocarcinoma tissues and normal colorectal tissues were taken and suppression subtractive hybridization (SSH) and cDNA microarray techniques were employed. From differentially expressed 86 expressed sequence tags (EST), 10 EST of the SSH were selected as seed sequence for bioinformatics analyses, semi-quantitative RT-PCR and PCR-sequencing. Each lane of semi-quantitative RT-PCR was analyzed by Q1 software.

RESULTS

Among these 10 EST, it has been found that ES274070, ES274071, ES274076 and ES274081 may play role in the onset of colorectal adenocarcinoma in human.

CONCLUSIONS

The ES274070, ES274071, ES274076 and ES274081 are related to the onset of human colorectal adenocarcinoma.

摘要

背景

本研究旨在探究人类结直肠癌中的一些新的病理基因。

方法

采集人类结直肠癌组织和正常结直肠组织，采用抑制性消减杂交（SSH）和cDNA微阵列技术。从差异表达的86个表达序列标签（EST）中，选择10个SSH的EST作为种子序列进行生物信息学分析、半定量RT-PCR和PCR测序。半定量RT-PCR的每条泳道用Q1软件进行分析。

结果

在这10个EST中，已发现ES274070、ES274071、ES274076和ES274081可能在人类结直肠癌的发病中起作用。

结论

ES274070、ES274071、ES274076和ES274081与人类结直肠癌的发病有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6032/5139827/211a53f749b0/gr-02-110-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6032/5139827/0da9376290b9/gr-02-110-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6032/5139827/845712c1ea8d/gr-02-110-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6032/5139827/211a53f749b0/gr-02-110-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6032/5139827/0da9376290b9/gr-02-110-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6032/5139827/845712c1ea8d/gr-02-110-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6032/5139827/211a53f749b0/gr-02-110-g003.jpg

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