Namieno T, Takeichi N, Dempo K, Mori M, Uchino J, Kobayashi H
Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
Nihon Geka Gakkai Zasshi. 1989 Jun;90(6):886-93.
We investigated LEC rats immunopathologically which spontaneously developed hepatitis to find out the genesis, in comparison with non-hepatitis LEA (Long Evans Agouti) rats. 1) Wet weights of the spleen and thymus of 6-week old LEC rats were significantly lighter than those of LEA rats of the same age. 2) Serum IgG (Immunoglobulin G) in LEC rats remained markedly low after the age of two months and IgG antibody formation to SRBC (Sheep Red Blood Cell) as detected by plaque assay was also significantly suppressed. On the other hand, IgM antibody formation to SRBC was significantly suppressed through serum IgM level in LEC rats was normal or rather increased. 3) Blastogenic responses of spleen cells to PHA and Con A were much more suppressed in LEC rats than in LEA rats. 4) Cytostatic activity of intraperitoneal macrophages against tumor cells was more evident in LEC rats than in LEA rats, but there was no difference in NK (natural killer) activity between the two rat strains. From these results, it is speculated that spontaneously hepatitis-developing LEC rats possess T and B cell deficiency (combined immunodeficiency) and that the increase of macrophage and NK cell activities are linked to the genesis of developing hepatitis.
我们对自发发生肝炎的LEC大鼠进行了免疫病理学研究，以找出其发病机制，并与无肝炎的LEA（Long Evans Agouti）大鼠进行比较。1）6周龄LEC大鼠的脾脏和胸腺湿重明显低于同龄LEA大鼠。2）LEC大鼠血清IgG（免疫球蛋白G）在2个月龄后仍显著降低，通过空斑试验检测到的对SRBC（绵羊红细胞）的IgG抗体形成也受到显著抑制。另一方面，尽管LEC大鼠血清IgM水平正常或有所升高，但对SRBC的IgM抗体形成仍受到显著抑制。3）LEC大鼠脾细胞对PHA和Con A的增殖反应比LEA大鼠受到更明显的抑制。4）LEC大鼠腹腔巨噬细胞对肿瘤细胞的细胞抑制活性比LEA大鼠更明显，但两种大鼠品系的NK（自然杀伤）活性没有差异。从这些结果推测，自发发生肝炎的LEC大鼠存在T细胞和B细胞缺陷（联合免疫缺陷），巨噬细胞和NK细胞活性的增加与肝炎的发生有关。
