Sun Meixiang, Wu Man, Liu Wen, Liu Huiying, Zhang Yezhong, Dai Jie
College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434023, China E-mail:
Water Sci Technol. 2016 Dec;74(11):2708-2716. doi: 10.2166/wst.2016.347.
A hybrid membrane bioreactor (HMBR) with biological band carriers (Reactor A) and an HMBR with suspended honeycomb carriers (Reactor B) were conducted in parallel to investigate the effects of different carriers on extracellular polymeric substances (EPS). Composition and concentration of EPS were examined by three-dimensional excitation-emission matrix (3DEEM) fluorescence spectra and parallel factor analysis (PARAFAC). 3DEEM spectra demonstrated that the main organic substances of the EPS in two reactors were protein-like, humic acid-like and fulvic acid-like substances. The fluorescence intensity (FI) indicated that the protein-like composition was dominant in EPS, and its intensity in reactor B was stronger than that in A (392.94 > 250.25). Results of the FI identified from the 3DEEM by PARAFAC showed that the EPS in two reactors included two humic acid-like compositions C1 (230, 320/406 nm), C2 (250, 360/440 nm) and one protein-like C4 (230, 280/340 nm), while C3 was fulvic acid-like (220/429 nm) and protein-like (230/357 nm) in reactor A and B, respectively. The proportion and FI of protein-like substances in reactor B were higher than that in A. Consequently, it was concluded that reactor A could control the membrane fouling effectively, compared with reactor B.
为了研究不同载体对胞外聚合物（EPS）的影响，进行了两个平行实验，分别是带有生物带载体的混合膜生物反应器（反应器A）和带有悬浮蜂窝载体的混合膜生物反应器（反应器B）。通过三维激发-发射矩阵（3DEEM）荧光光谱和平行因子分析（PARAFAC）来检测EPS的组成和浓度。3DEEM光谱表明，两个反应器中EPS的主要有机物质是类蛋白质、类腐殖酸和类富里酸物质。荧光强度（FI）表明，EPS中类蛋白质成分占主导，且其在反应器B中的强度高于反应器A（392.94 > 250.25）。通过PARAFAC从3DEEM中识别出的FI结果表明，两个反应器中的EPS包括两种类腐殖酸成分C1（230，320/406 nm）、C2（250，360/440 nm）和一种类蛋白质C4（230，280/340 nm）, 而C3在反应器A和B中分别为类富里酸（220/429 nm）和类蛋白质（230/357 nm）。反应器B中类蛋白质物质的比例和FI高于反应器A。因此，得出结论：与反应器B相比，反应器A能更有效地控制膜污染。
