SKKU Advanced Institute of Nanotechnology (SAINT), School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Biomater Sci. 2017 Jan 31;5(2):285-294. doi: 10.1039/c6bm00711b.
Mesenchymal stem cells (MSCs) can ameliorate renal injury and accelerate repair of acute kidney injury. Herein, we developed a collagen/poly(γ-glutamic acid) (γ-PGA) hydrogel as an injectable scaffold for the delivery of mouse MSCs (mMSCs) and anti-oxidant drugs into injured sites. By the introduction of γ-PGA into conventional collagen, the viscosity of collagen was reduced at ambient temperature for easy handling, while the elastic and viscous moduli of collagen were increased and a new porous structure was generated near body temperature. When in situ gel-forming collagen/γ-PGA hydrogels loaded with mMSCs and α-lipoic acid (LA) were administered to a mouse model of renal dysfunction, they significantly attenuated the level of blood urea nitrogen and creatinine, which resulted from the increased retention of therapeutic mMSCs and the controlled release of anti-oxidant drugs at the injured site. These findings suggested that this novel type of hydrogel could be applied as an injectable scaffold for use in regenerative medicine.
间充质干细胞 (MSCs) 可减轻肾损伤并加速急性肾损伤的修复。在此，我们开发了一种胶原/聚 (γ-谷氨酸) (γ-PGA) 水凝胶作为可注射支架，用于将小鼠间充质干细胞 (mMSCs) 和抗氧化药物递送到损伤部位。通过将 γ-PGA 引入常规胶原中，胶原在环境温度下的粘度降低，便于处理，同时胶原的弹性和粘性模量增加，并在接近体温时产生新的多孔结构。当将负载 mMSCs 和 α-硫辛酸 (LA) 的原位形成凝胶的胶原/γ-PGA 水凝胶施用于肾功能障碍的小鼠模型时，它们显著降低了血液中尿素氮和肌酐的水平，这是由于治疗性 mMSCs 的保留增加和抗氧化药物在损伤部位的控释所致。这些发现表明，这种新型水凝胶可以用作可注射支架，用于再生医学。
