Gerson Julia E, Sengupta Urmi, Kayed Rakez
George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555-1045, USA.
Methods Mol Biol. 2017;1523:141-157. doi: 10.1007/978-1-4939-6598-4_9.
Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.
在许多神经退行性疾病中，tau寡聚体已被证明是主要的毒性tau蛋白种类。为了在体外和体内研究tau寡聚体，我们已经建立了可靠制备、分离和检测tau寡聚体的方法。以下详细介绍tau寡聚体的接种方法、从组织中分离tau寡聚体以及通过生化和免疫组化方法使用tau寡聚体特异性抗体检测tau寡聚体的方法。
