Teo Anges, Lee Sung Je, Goh Kelvin K T, Wolber Frances M
School of Food and Nutrition, Massey University, Auckland, New Zealand; School of Food and Nutrition, Massey University, Palmerston North, New Zealand.
School of Food and Nutrition, Massey University, Auckland, New Zealand.
Food Chem. 2017 Apr 15;221:1269-1276. doi: 10.1016/j.foodchem.2016.11.030. Epub 2016 Nov 9.
The particle size and lutein encapsulation efficiency of nanoemulsions prepared by emulsification and solvent evaporation method were 68.8±0.3nm and 80.7±0.8%, respectively, whereas they were 147.3±0.6nm and 86.3±0.3% for conventional emulsions. All the emulsions had no change in their particle size during storage (28days at 5, 20 and 40°C) but their lutein content and emulsion colour decreased, especially at 40°C. The lutein emulsions were analysed using MTT assay on the gut enterocyte cell line Caco-2 and they showed no toxicity as the cell viability was more than 80% at 10times or higher dilution after 24h of incubation. However, there was a higher cellular uptake of lutein by Caco-2 cells in nanoemulsions (872.9±88.3pmol/mgprotein) than conventional emulsions (329.5±214.6pmol/mgprotein). The results of this study indicated that nanoemulsions can be used as a delivery system to improve the cellular uptake of lutein.
通过乳化和溶剂蒸发法制备的纳米乳液的粒径和叶黄素包封率分别为68.8±0.3nm和80.7±0.8%，而常规乳液的粒径和叶黄素包封率分别为147.3±0.6nm和86.3±0.3%。在储存期间（5、20和40°C下储存28天），所有乳液的粒径均无变化，但它们的叶黄素含量和乳液颜色有所下降，尤其是在40°C时。使用MTT法对肠道肠上皮细胞系Caco-2进行分析，结果表明叶黄素乳液无毒性，因为在孵育24小时后，在10倍或更高稀释度下细胞活力超过80%。然而，与常规乳液（329.5±214.6pmol/mg蛋白质）相比，纳米乳液中Caco-2细胞对叶黄素的细胞摄取量更高（872.9±88.3pmol/mg蛋白质）。本研究结果表明，纳米乳液可作为一种递送系统来提高叶黄素的细胞摄取量。
