绿原酸-明胶的制备与表征：一种用于涂膜保鲜的生物活性薄膜
Preparation and characterisation of Chlorogenic acid-gelatin: A type of biologically active film for coating preservation.
作者信息
Fu Shalu, Wu Chunhua, Wu Tiantian, Yu Haixia, Yang Shuibing, Hu Yaqin
机构信息
College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310029, China; Marine Research Center of Zhoushan, Zhejiang University, Zhoushan 316021, China.
College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310029, China.
出版信息
Food Chem. 2017 Apr 15;221:657-663. doi: 10.1016/j.foodchem.2016.11.123. Epub 2016 Nov 23.
Chlorogenic acid (CGA), a type of plant polyphenol, was conjugated onto gelatin (Gel) to prepare a novel coating material for the preservation of fresh seafood. The optimal reaction molar ratio of CGA to gelatin (4:1) was determined according to the CGA content in the CGA-Gel conjugate. CGA was confirmed to be successfully conjugated onto gelatin by H nuclear magnetic resonance and Fourier transform-infrared spectroscopy. The antioxidant activity of CGA-Gel was proven to be higher than that of the free CGA in 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical scavenging, hydrogen peroxide scavenging, ferric ion reducing power and lipid oxidation assays. The minimum inhibitory concentrations (MIC) of CGA against Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes and Staphylococcus aureus were 1, 1, 2 and 2mg/mL, respectively. The antibacterial activity of CGA-Gel was unaffected by conjugation.
绿原酸（CGA）是一种植物多酚，将其与明胶（Gel）结合，制备出一种用于新鲜海产品保鲜的新型涂层材料。根据CGA - Gel共轭物中的CGA含量，确定了CGA与明胶的最佳反应摩尔比（4:1）。通过氢核磁共振和傅里叶变换红外光谱证实CGA成功地与明胶结合。在2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）自由基清除、过氧化氢清除、铁离子还原能力和脂质氧化试验中，CGA - Gel的抗氧化活性被证明高于游离CGA。CGA对大肠杆菌、铜绿假单胞菌、单核细胞增生李斯特菌和金黄色葡萄球菌的最小抑菌浓度（MIC）分别为1、1、2和2mg/mL。CGA - Gel的抗菌活性不受结合的影响。
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