Department of Nutrition, School of Public Health, University of São Paulo - USP, Avenida Doutor Arnaldo, 715, Cerqueira César, CEP 01246-904 São Paulo, SP, Brazil.
Food Chem. 2017 Apr 15;221:809-814. doi: 10.1016/j.foodchem.2016.11.098. Epub 2016 Nov 22.
Among the different food categories, the oils and fats are important sources of exposure to polycyclic aromatic hydrocarbons (PAHs), a group of organic chemical contaminants. The use of a validated method is essential to obtain reliable analytical results since the legislation establishes maximum limits in different foods. The objective of this study was to optimize and validate a method for the quantification of four PAHs [benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(a)pyrene] in vegetable oils. The samples were submitted to liquid-liquid extraction, followed by solid-phase extraction, and analyzed by ultra-high performance liquid chromatography. Under the optimized conditions, the validation parameters were evaluated according to the INMETRO Guidelines: linearity (r2 >0.99), selectivity (no matrix interference), limits of detection (0.08-0.30μgkg) and quantification (0.25-1.00μgkg), recovery (80.13-100.04%), repeatability and intermediate precision (<10% RSD). The method was found to be adequate for routine analysis of PAHs in the vegetable oils evaluated.
在不同的食物类别中，油和脂肪是多环芳烃（PAHs）暴露的重要来源，PAHs 是一组有机化学污染物。由于法规在不同食品中设定了最大限量，因此使用经过验证的方法对于获得可靠的分析结果至关重要。本研究的目的是优化和验证一种用于定量分析植物油中四种 PAHs（苯并[a]蒽、䓛、苯并[b]荧蒽、苯并[a]芘）的方法。样品经过液-液萃取，然后进行固相萃取，最后通过超高效液相色谱进行分析。在优化条件下，根据 INMETRO 指南评估验证参数：线性（r2>0.99）、选择性（无基质干扰）、检测限（0.08-0.30μgkg）和定量限（0.25-1.00μgkg）、回收率（80.13-100.04%）、重复性和中间精密度（RSD<10%）。该方法适用于所评估植物油中 PAHs 的常规分析。
