Ma Yongjie, Dong Wenbin, Bao Hongliang, Fang Yue, Fan Cheng
College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Weiyang University Circle, Xi'an 710021, PR China; Mathematical and Sciences College, LouYang Normal University, University Street, Luoyang 471022, PR China.
College of Food and Biological Engineering, Shaanxi University of Science and Technology, Weiyang University Circle, Xi'an 710021, PR China.
Food Chem. 2017 Apr 15;221:898-906. doi: 10.1016/j.foodchem.2016.11.076. Epub 2016 Nov 19.
This paper proposed a nonlinear chemical fingerprint method for simultaneous determination of urea and melamine in milk powder using "H+Ce+BrO+malonic acid" as reaction system. A multiple linear relationship was obtained between the adulterants content in milk powder and inductive time of corresponding mixed milk powder. System analysis model established with classical least squares (CLS) method was then used to calculate the content of urea and melamine in milk powder. The method was successfully applied to milk powder samples and had good recoveries in the range of 99.17-100.25%, with the relative standard deviation (RSD) in the range of 0.60-4.12%. The limits of detection for urea and melamine were 0.33μg·g and 0.05μg·g, respectively. The limits of quantification were 1.11μg·g and 0.18μg·g, respectively. The results indicated that the new method was feasible and had the advantages of low cost, simple operation and without pretreatment of samples.
本文提出了一种以“H⁺ + Ce + BrO₃⁻ + 丙二酸”为反应体系的非线性化学指纹图谱法，用于同时测定奶粉中的尿素和三聚氰胺。获得了奶粉中掺假物含量与相应混合奶粉诱导时间之间的多元线性关系。然后使用经典最小二乘法（CLS）建立的系统分析模型来计算奶粉中尿素和三聚氰胺的含量。该方法成功应用于奶粉样品，回收率良好，在99.17 - 100.25%范围内，相对标准偏差（RSD）在0.60 - 4.12%范围内。尿素和三聚氰胺的检测限分别为0.33μg·g和0.05μg·g。定量限分别为1.11μg·g和0.18μg·g。结果表明，该新方法可行，具有成本低、操作简单且无需样品预处理的优点。
