Brunet Marcel Durante, Araujo Cristiano Miranda de, Johann Aline Cristina Batista Rodrigues, Camargo Elisa Souza, Tanaka Orlando Motohiro, Guariza Odilon
Graduate Program in Dentistry, Orthodontics, School of Health and Biosciences, PUCPR - Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.
Graduate Program in Dentistry, Histopathology, School of Health and Biosciences, PUCPR - Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.
Braz Dent J. 2016 Sep-Oct;27(5):515-523. doi: 10.1590/0103-6440201600966.
The purpose of this study was to assess the effects of the administration of zoledronic acid (ZA) during orthodontic movement in rats. A hundred and twenty male Wistar rats were applied force of 30 cN with spring closed nickel-titanium to move the upper right first molar to mesial. In the Control Movement group (CM), only tooth movement was performed; the Control Acid Zoledronic group (CAZ) received a single dose (0.1 mg/kg) of ZA; the Experimental Acid Zoledronic group (EAZ) received a single dose (0.1 mg/kg) one week prior to the start of tooth movement; and the Control Without movement group (CWM) that received no drug and without application of tooth movement. The animals were euthanized after 3, 7 and 14 days. Tooth movement was measured using a caliper, the number of osteoclasts using TRAP staining, the expression of mature and immature collagen using picrosirius staining, and the presence of hyaline areas and root resorption using HE. The data were compared using two-way ANOVA, Tukey HSD, Games-Howell and chi-squared test, at the 5% significance level. It was observed a smaller number of osteoclasts and greater percentage of hyaline area in the EAZ group. There was no difference among the groups regarding bone remodeling, root resorption and tooth movement for all observed times.
本研究的目的是评估在大鼠正畸移动过程中给予唑来膦酸（ZA）的效果。将120只雄性Wistar大鼠用闭合弹簧镍钛丝施加30 cN的力，使右上第一磨牙向近中移动。在对照移动组（CM）中，仅进行牙齿移动；对照唑来膦酸组（CAZ）接受单剂量（0.1 mg/kg）的ZA；实验唑来膦酸组（EAZ）在牙齿移动开始前一周接受单剂量（0.1 mg/kg）；未移动对照组（CWM）不接受药物且不进行牙齿移动。在3、7和14天后对动物实施安乐死。使用卡尺测量牙齿移动，使用抗酒石酸酸性磷酸酶（TRAP）染色测量破骨细胞数量，使用天狼星红染色测量成熟和未成熟胶原蛋白的表达，使用苏木精-伊红（HE）染色观察透明区和牙根吸收情况。数据采用双向方差分析、Tukey HSD检验、Games-Howell检验和卡方检验进行比较，显著性水平为5%。观察到EAZ组破骨细胞数量较少，透明区百分比更高。在所有观察时间内，各组在骨重塑、牙根吸收和牙齿移动方面均无差异。
