Franzoni J S, Soares F M P, Zaniboni E, Vedovello Filho M, Santamaria M P, Dos Santos G M T, Esquisatto M A M, Felonato M, Mendonca F A S, Franzini C M, Santamaria M
Graduate Program of Orthodontics, Heminio Ometto University Center, UNIARARAS, Sao Paulo, Brazil.
Graduate Program of Biomedical Sciences, Heminio Ometto University Center, UNIARARAS, Sao Paulo, Brazil.
Orthod Craniofac Res. 2017 Aug;20(3):164-169. doi: 10.1111/ocr.12192. Epub 2017 Jun 27.
To evaluate orthodontic tooth movement (OTM) in rats treated with two types of bisphosphonates (BPs), alendronate sodium (A) and zoledronic acid (Z).
In all, 15 male Wistar rats were randomly divided into three groups. Group OTM+A: orthodontic tooth movement and subcutaneous administration of alendronate sodium (2.5 mg/kg); Group OTM+Z: orthodontic tooth movement and subcutaneous administration of zoledronic acid (0.02 mg/kg), and Group OTM: orthodontic tooth movement and subcutaneous injection of saline. The BPs were administered once a day during 25 days before OTM started and during 10 days of OTM. The left upper first molar was moved with a stainless-steel closed coil spring which delivered an initial force of 0.4N. OTM was measured with a digital caliper comparing the moved and the contralateral side. The histomorphometric analysis counted the number of osteoclasts, inflammatory cells, blood vessels and fibroblasts (n/10 m ) in periodontal ligament (PDL) of the distobuccal root.
A reduction of 58.3% of OTM was found in Group OTM+A and 99.6% in Group OTM+Z, when compared with Group OTM. There was a significant decrease of osteoclasts and inflammatory cells in BP-treated groups. Blood vessels and fibroblastic cells decreased mainly in Group OTM+Z.
Alendronate sodium and zoledronic acid have similar effects on the periodontal tissue during orthodontic treatment in rats. Especially, zoledronic acid can affect orthodontic tooth movement.
评估用两种双膦酸盐（BPs），阿仑膦酸钠（A）和唑来膦酸（Z）治疗的大鼠的正畸牙齿移动（OTM）情况。
总共15只雄性Wistar大鼠被随机分为三组。OTM+A组：正畸牙齿移动并皮下注射阿仑膦酸钠（2.5毫克/千克）；OTM+Z组：正畸牙齿移动并皮下注射唑来膦酸（0.02毫克/千克），以及OTM组：正畸牙齿移动并皮下注射生理盐水。在OTM开始前的25天以及OTM进行的10天期间，每天给药一次BPs。用不锈钢闭合螺旋弹簧移动左上第一磨牙，该弹簧施加的初始力为0.4N。用数字卡尺测量OTM，比较移动侧和对侧。组织形态计量学分析计算远中颊根牙周膜（PDL）中破骨细胞、炎性细胞、血管和成纤维细胞的数量（每10微米中的数量）。
与OTM组相比，OTM+A组的OTM减少了58.3%，OTM+Z组减少了99.6%。BP治疗组的破骨细胞和炎性细胞显著减少。血管和成纤维细胞主要在OTM+Z组减少。
阿仑膦酸钠和唑来膦酸在大鼠正畸治疗期间对牙周组织有相似的影响。特别是，唑来膦酸会影响正畸牙齿移动。
