Hong Zehui, Li Hui, Li Lili, Wang Weilong, Xu Ting
Department of Genetics and Developmental Biology, Medical School of Southeast University, The Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Nanjing 210009, Jiangsu, China.
Institute of Life Sciences, The Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, Jiangsu, China.
Cancer Biomark. 2017;18(2):125-131. doi: 10.3233/CBM-160003.
UTX and JMJD3 are recently identified histone H3 lysine 27 (H3K27) demethylases. Many studies have shown aberrant H3K27 trimethylation (H3K27me3) levels widely exist in multiple cancers, and that altered H3K27me3 levels are correlated with tumorigenesis and tumor progression. To investigate expression patterns of UTX and JMJD3 genes in renal cell carcinoma (RCC) and bladder cancer and the relationship between gene expression and tumor development.
Samples were collected from 35 patients with RCC and 21 patients with bladder cancer and qRT-PCR was performed.
By comparing with adjacent normal tissues, the expression of JMJD3 (10/21 = 47.62%) and UTX (10/21 = 47.62%) were significantly upregulated in bladder cancer tissues and the expression of JMJD3 (15/35 = 42.86%) was significantly downregulated in RCC tissues. Stratified analyses revealed that upregulated expression of JMJD3 was significantly associated with poorly differentiated tumor nuclear grade (p= 0.005) and advanced clinical stage (p= 0.043) in the bladder cancer group, while downregulated expression of JMJD3 was significantly associated with advanced clinical stage (p= 0.045) and poorly differentiated tumor nuclear grade (p= 0.011) in the RCC group.
These results suggest JMJD3 could be a hallmark and is involved in the development of RCC and bladder cancers. The potential role of H3K27 demethylases as biomarkers needs further investigations.
UTX和JMJD3是最近发现的组蛋白H3赖氨酸27（H3K27）去甲基化酶。许多研究表明，异常的H3K27三甲基化（H3K27me3）水平广泛存在于多种癌症中，且H3K27me3水平的改变与肿瘤发生和肿瘤进展相关。本研究旨在探讨UTX和JMJD3基因在肾细胞癌（RCC）和膀胱癌中的表达模式，以及基因表达与肿瘤发展之间的关系。
收集35例RCC患者和21例膀胱癌患者的样本，并进行qRT-PCR检测。
与癌旁正常组织相比，JMJD3（10/21 = 47.62%）和UTX（10/21 = 47.62%）在膀胱癌组织中的表达显著上调，而JMJD3（15/35 = 42.86%）在RCC组织中的表达显著下调。分层分析显示，在膀胱癌组中，JMJD3表达上调与肿瘤核分级差（p = 0.005）和临床晚期（p = 0.043）显著相关；而在RCC组中，JMJD3表达下调与临床晚期（p = 0.045）和肿瘤核分级差（p = 0.011）显著相关。
这些结果表明JMJD3可能是RCC和膀胱癌发生发展的一个标志。H3K27去甲基化酶作为生物标志物的潜在作用需要进一步研究。
