Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:1028-1036. doi: 10.1016/j.msec.2016.11.055. Epub 2016 Nov 16.
As a prerequisite of gene delivery in living cells, DNA condensation has attracted more and more attention. In order to improve the efficiencies of polyamine-β-cyclodextrin-based cationic polyrotaxanes (PR-EDA and PR-DETA) as DNA condensation materials, we have designed and prepared two novel scutellarin-grafted cationic polyrotaxanes (PR-EDA-SCU and PR-DETA-SCU), in which scutellarins (SCU), the planar molecules, were conjugated on the cyclodextrin molecules of PR-EDA and PR-DETA. These materials were characterized by 1D and 2D NMR, XRD, TG and DSC. The electrophoresis assays showed that pDNA condensation efficiencies of PR-EDA and PR-DETA were better than that of PR-EDA and PR-DETA. The complexes of PR-EDA, PR-DETA, PR-EDA-SCU and PR-DETA-SCU with pDNA were further investigated by zeta potential and atomic force microscopy analysis. The results indicated that the planar structure of SCU played an important role in improvement of pDNA condensation efficiencies of PR-EDA-SCU and PR-DETA-SCU. The satisfactory pDNA condensation abilities of PR-EDA-SCU and PR-DETA-SCU could be helpful in designing non-viral gene delivery vectors to control gene expression and delivery.
作为活体细胞内基因传递的前提条件，DNA 凝聚受到了越来越多的关注。为了提高多胺-β-环糊精基阳离子聚轮烷（PR-EDA 和 PR-DETA）作为 DNA 凝聚材料的效率，我们设计并制备了两种新型的小檗碱接枝阳离子聚轮烷（PR-EDA-SCU 和 PR-DETA-SCU），其中小檗碱（SCU）为平面分子，接枝在 PR-EDA 和 PR-DETA 的环糊精分子上。这些材料通过 1D 和 2D NMR、XRD、TG 和 DSC 进行了表征。电泳实验表明，PR-EDA 和 PR-DETA 的 pDNA 凝聚效率优于 PR-EDA 和 PR-DETA。还通过 Zeta 电位和原子力显微镜分析进一步研究了 PR-EDA、PR-DETA、PR-EDA-SCU 和 PR-DETA-SCU 与 pDNA 的复合物。结果表明，SCU 的平面结构对提高 PR-EDA-SCU 和 PR-DETA-SCU 的 pDNA 凝聚效率起着重要作用。PR-EDA-SCU 和 PR-DETA-SCU 令人满意的 pDNA 凝聚能力有助于设计非病毒基因传递载体来控制基因表达和传递。
