Merli M, Bergui G, Cattani C, Berti F, Marchetti M
Riv Eur Sci Med Farmacol. 1989 Apr;11(2):175-82.
The effect of fructose-1,6-diphosphate (FDP) on the recovery of post-arrest cardiac performance was assessed in isolated rabbit heart preparations subjected to cold ischemic cardioplegic arrest (CICA). Twenty-eight hearts were perfused with Krebs-Henseleit (KHBS) solution followed by 40 minutes of CICA. After CICA normothermic reperfusion with KHBS was restarted. The experimental preparations were divided in four groups of seven hearts each: one group served as control and the other three were perfused with KHBS supplemented with FDP (500 mg/l) given before CICA, after CICA or at both study points. Left ventricular end-diastolic pressure, left ventricular systolic pressure, dP/dt and perfusion pressure were measured at different pre- and post-arrest phases. Time analysis of recovery was also performed. The results demonstrated that FDP supplied prior to and after CICA: prevents the ventricular wall rigidity induced by the ischemic arrest, improves cardiac contractile force both in the basal condition and after cardioplegia, reduces the perfusion pressure, reduces the time to recovery of cardiac contractility. Our findings in the isolated rabbit heart are consistent with published data suggesting FDP may limit the impairment of cardiac dynamics induced by ischemia and improve the recovery after cardiac arrest.
在经历冷缺血心脏停搏（CICA）的离体兔心标本中，评估了1,6 - 二磷酸果糖（FDP）对心脏停搏后心脏功能恢复的影响。28颗心脏先用克雷布斯 - 亨泽莱特（KHBS）溶液灌注，随后进行40分钟的CICA。CICA后，重新开始用KHBS进行常温再灌注。实验标本分为四组，每组七颗心脏：一组作为对照，其他三组在CICA前、CICA后或两个研究点均用补充了FDP（500 mg/l）的KHBS灌注。在心脏停搏前后的不同阶段测量左心室舒张末期压力、左心室收缩压、dP/dt和灌注压力。还进行了恢复的时间分析。结果表明，在CICA前后给予FDP：可防止缺血性停搏引起的心室壁僵硬，改善基础状态和心脏停搏后的心脏收缩力，降低灌注压力，缩短心脏收缩力恢复时间。我们在离体兔心中的研究结果与已发表的数据一致，表明FDP可能限制缺血引起的心脏动力学损害，并改善心脏停搏后的恢复。
