Ning Zhi-Qiang, Lu Hai-Lin, Chen Chao, Wang Lin, Cai Wei, Li Yan, Cao Ting-Hua, Zhu Jing, Shu Yong-Qian, Shen Hua
Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
Institute of Medcine, University of Zhengzhou, Henan Province, 450000, China.
Oncotarget. 2017 Jan 17;8(3):4572-4581. doi: 10.18632/oncotarget.13944.
MicroRNAs (miRNAs) play critical roles in variousbiological processes,including malignancy. Here, we demonstrated that miR-30e levels were markedly reduced in human lung carcinoma specimens in comparisonwith adjacent normal tissues.In addition, miR-30eamounts were starkly lower in the resistant PC9/gefitinib (PC9G) cancer cells compared with PC9 cells. Meanwhile, miR-30eoverexpression inPC9G cells resulted in reduced cell proliferation and migration,reversing drug resistance to gefitinib.Conversely,miR-30e silencing in PC9 cells increased proliferation as well as migration, and conferred resistance to gefitinib.Moreover, HOXA1, which was identified asa new miR-30etarget, plays important roles in regulating cell fate, early developmental patterns and organogenesis.Importantly, miR-30ealso inhibited PC9G growth in vivo. Taken together, these findings demonstrated that miR-30eshould be considered a tumor suppressor miRNA, which could be used in treatinghuman lung cancer.
微小RNA（miRNA）在包括恶性肿瘤在内的各种生物学过程中发挥着关键作用。在此，我们证明，与相邻正常组织相比，人肺癌标本中miR-30e的水平显著降低。此外，与PC9细胞相比，耐药的PC9/吉非替尼（PC9G）癌细胞中miR-30e的含量明显更低。同时，PC9G细胞中miR-30e的过表达导致细胞增殖和迁移减少，逆转了对吉非替尼的耐药性。相反，PC9细胞中miR-30e的沉默增加了增殖和迁移，并赋予了对吉非替尼的耐药性。此外，HOXA1被确定为一个新的miR-30e靶点，在调节细胞命运、早期发育模式和器官发生中发挥重要作用。重要的是，miR-30e在体内也抑制了PC9G的生长。综上所述，这些发现表明miR-30e应被视为一种肿瘤抑制性miRNA，可用于治疗人类肺癌。
