Rabelo Kíssila, Trugilho Monique R O, Costa Simone M, Ferreira André T S, Carvalho Paulo C, Perales Jonas, Alves Ada M B
Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
Laboratory of Toxinology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
Data Brief. 2016 Dec 6;10:248-263. doi: 10.1016/j.dib.2016.11.083. eCollection 2017 Feb.
The data supplied in this article are related to the research article entitled "The effect of the dengue non-structural 1 protein expression over the HepG2 cell proteins in a proteomic approach" (K. Rabelo, M.R. Trugillo, S.M. Costa, B.A. Pereira, O.C. Moreira, A.T. Ferreira et al., 2016) [1]. The present article provides the inventory of peptides and proteins mapped in a hepatocyte cell line (HepG2) by mass spectrometry in the presence of the non-structural protein 1 (NS1) of Dengue 2 virus (DENV2). Cells were transfected with pcENS1 plasmid, which encodes the DENV2 NS1 protein, or the controls pcDNA3 (negative control) or pMAXGFP, encoding the green fluorescent protein (GFP), a protein unrelated to dengue. Differentially abundant protein lists were obtained by comparing cells transfected with pcENS1 and controls.
本文提供的数据与题为《蛋白质组学方法研究登革热非结构蛋白1表达对HepG2细胞蛋白质的影响》（K. 拉贝洛、M.R. 特鲁希略、S.M. 科斯塔、B.A. 佩雷拉、O.C. 莫雷拉、A.T. 费雷拉等人，2016年）[1]的研究论文相关。本文提供了在登革热2型病毒（DENV2）非结构蛋白1（NS1）存在的情况下，通过质谱法在肝细胞系（HepG2）中鉴定出的肽段和蛋白质清单。用编码DENV2 NS1蛋白的pcENS1质粒，或对照质粒pcDNA3（阴性对照）或编码绿色荧光蛋白（GFP，一种与登革热无关的蛋白）的pMAXGFP转染细胞。通过比较用pcENS1转染的细胞和对照细胞，获得了差异丰度蛋白质列表。
