过表达 VEGF 的乳腺癌异种移植物中的血管生成分泌组。
The Angiogenic Secretome in VEGF overexpressing Breast Cancer Xenografts.
机构信息
JHU ICMIC Program, Division of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Systems Biology Laboratory, Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
出版信息
Sci Rep. 2016 Dec 20;6:39460. doi: 10.1038/srep39460.
The plasticity of cancer cells and the fluidity of the tumor microenvironment continue to present major challenges in the comprehensive understanding of cancer that is essential to design effective treatments. The tumor interstitial fluid (TIF) encompasses the secretome and holds the key to several of the phenotypic characteristics of cancer. Difficulties in sampling this fluid have resulted in limited characterization of its components. Here we have sampled TIF from triple negative and estrogen receptor (ER)-positive human breast tumor xenografts with or without VEGF overexpression. Angiogenesis-related factors were characterized in the TIF and plasma, to understand the relationship between the TIF and plasma secretomes. Clear differences were observed between the TIF and plasma angiogenic secretomes in triple negative MDA-MB-231 breast cancer xenografts compared to ER-positive MCF-7 xenografts with or without VEGF overexpression that provide new insights into TIF components and the role of VEGF in modifying the angiogenic secretome.
肿瘤细胞的可塑性和肿瘤微环境的流动性，继续对全面了解癌症构成主要挑战，而癌症的全面了解对于设计有效的治疗方法至关重要。肿瘤间质液（TIF）包含了分泌组，是癌症的几种表型特征的关键。由于难以对这种液体进行采样，其成分的特征描述受到了限制。在这里，我们从过表达 VEGF 的三阴性和雌激素受体（ER）阳性人乳腺癌异种移植瘤中采样 TIF。对 TIF 和血浆中的血管生成相关因子进行了特征分析，以了解 TIF 和血浆分泌组之间的关系。与过表达 VEGF 的 ER 阳性 MCF-7 异种移植瘤相比，在三阴性 MDA-MB-231 乳腺癌异种移植瘤中，TIF 和血浆的血管生成分泌组之间存在明显差异，这为 TIF 成分和 VEGF 在改变血管生成分泌组中的作用提供了新的见解。
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