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Specific capture of ABH blood group antigens of the red cell or body fluids by double antibody sandwich-ELISA.

作者信息

Sagisaka K, Fletcher S M, Katsura S, Yokoi T

机构信息

Department of Forensic Medicine, Tohoku University School of Medicine, Sendai.

出版信息

Tohoku J Exp Med. 1989 Jul;158(3):211-9. doi: 10.1620/tjem.158.211.

DOI:10.1620/tjem.158.211
PMID:2799807
Abstract

A double antibody sandwich-ELISA method for the detection of the ABH blood group of each constituent of mixed stains is described. Extracts from mixed stains were applied to microtitration plates coated with rabbit polyclonal antisera to red cells or body fluids. ABH antigens in body fluid stains which were captured by the polyclonal antibodies were detected by monoclonal anti-A and -B and enzyme-conjugated anti-mouse immunoglobulin. By this procedure, ABH antigens of only saliva, semen or red cells could be detected from mixed stains, but no ABH antigen capture activity was observed using anti-sweat, -milk, -vaginal secretion, -erythrocyte membrane and -band 3 antibodies.

摘要

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引用本文的文献

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Production and characterization of a monoclonal antibody to ABH-carrying alpha 2-seminoglycoprotein for ABO grouping of semen by ELISA.用于通过酶联免疫吸附测定法对精液进行ABO血型分组的抗携带ABH的α2-半乳糖蛋白单克隆抗体的制备与鉴定
Int J Legal Med. 1991;104(3):149-52. doi: 10.1007/BF01369720.

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