唾液、尿液和毛发中皮质醇和可的松的个体内稳定性以及皮质醇与可的松的比率。
Intraindividual stability of cortisol and cortisone and the ratio of cortisol to cortisone in saliva, urine and hair.
作者信息
Zhang Quan, Chen Zheng, Chen Shenghuo, Xu Youyun, Deng Huihua
机构信息
Key Laboratory of Child Development and Learning Science (Southeast University), Ministry of Education, and Institute of Child Development and Education, Research Center for Learning Science, Southeast University, Nanjing 210096, China.
Key Laboratory of Child Development and Learning Science (Southeast University), Ministry of Education, and Institute of Child Development and Education, Research Center for Learning Science, Southeast University, Nanjing 210096, China; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China.
出版信息
Steroids. 2017 Feb;118:61-67. doi: 10.1016/j.steroids.2016.12.008. Epub 2016 Dec 18.
BACKGROUND
Cortisol, cortisone and the ratio of cortisol to cortisone in saliva, urine and hair are acute, short-term and long-term biomarkers to reliably assess the activity of hypothalamic-pituitary-adrenal (HPA) axis and 11β-hydroxysteroid dehydrogenase (11β-HSD). One key issue is whether these biomarkers have intraindividual relative stability. Salivary, urinary and hair cortisol was proven to show considerable long-term intraindividual relative stability. However, currently unknown is whether cortisone and the ratio in saliva, urine and hair show intraindividual relative stability.
METHODS
The present study utilized a longitudinal design to validate long-term stability within two weeks of three biomarkers in saliva and urine, and long-term stability within twelve months of three hair biomarkers. Salivary, urinary and hair steroids were measured with high performance liquid chromatography tandem mass spectrometry.
RESULTS
Three biomarkers in urine and hair showed moderate test-retest correlations with coefficient (r) ranging between 0.22 and 0.56 and good multiple-test consistencies with coefficient of intraclass correlation (ICC) ranging between 0.42 and 0.67. Three single-point salivary biomarkers showed weak to moderate test-retest correlations (r's between 0.01 and 0.38) and poor to fair multiple-test consistencies (ICC's between 0.29 and 0.53) within two weeks. Three single-day salivary biomarkers showed moderate test-retest correlations (r's between 0.23 and 0.53) and good multiple-test consistencies (ICC's between 0.56 and 0.66) within two weeks.
CONCLUSIONS
Three biomarkers in urine and hair showed moderate long-term intraindividual relative stability. Three single-point salivary biomarkers showed weak to moderate short-term and long-term intraindividual relative stability, but three single-day salivary biomarkers showed moderate short-term and long-term intraindividual relative stability.
背景
唾液、尿液和毛发中的皮质醇、可的松以及皮质醇与可的松的比值是可靠评估下丘脑-垂体-肾上腺（HPA）轴和11β-羟基类固醇脱氢酶（11β-HSD）活性的急性、短期和长期生物标志物。一个关键问题是这些生物标志物是否具有个体内相对稳定性。唾液、尿液和毛发中的皮质醇已被证明具有相当程度的长期个体内相对稳定性。然而，目前尚不清楚唾液、尿液和毛发中的可的松及其比值是否具有个体内相对稳定性。
方法
本研究采用纵向设计，以验证唾液和尿液中三种生物标志物在两周内的长期稳定性，以及毛发中三种生物标志物在十二个月内的长期稳定性。唾液、尿液和毛发中的类固醇通过高效液相色谱串联质谱法进行测定。
结果
尿液和毛发中的三种生物标志物显示出中等程度的重测相关性，相关系数（r）在0.22至0.56之间，组内相关系数（ICC）在0.42至0.67之间，具有良好的多次测试一致性。三种单点唾液生物标志物在两周内显示出弱至中等程度的重测相关性（r在0.01至0.38之间），多次测试一致性较差至中等（ICC在0.29至0.53之间）。三种单日唾液生物标志物在两周内显示出中等程度的重测相关性（r在0.23至0.53之间），多次测试一致性良好（ICC在0.56至0.66之间）。
结论
尿液和毛发中的三种生物标志物显示出中等程度的长期个体内相对稳定性。三种单点唾液生物标志物显示出弱至中等程度的短期和长期个体内相对稳定性，但三种单日唾液生物标志物显示出中等程度的短期和长期个体内相对稳定性。
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