通过代谢工程减少解淀粉芽孢杆菌中支链短链脂肪酸的形成。
Decreased formation of branched-chain short fatty acids in Bacillus amyloliquefaciens by metabolic engineering.
作者信息
Chen Yangyang, Liu Mengjie, Chen Shouwen, Wei Xuetuan
机构信息
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
出版信息
Biotechnol Lett. 2017 Apr;39(4):529-533. doi: 10.1007/s10529-016-2270-5. Epub 2016 Dec 20.
OBJECTIVES
To reduce the unpleasant odor during 1-deoxynojirimycin (DNJ) production, the genes of leucine dehydrogenase (bcd) and phosphate butryltransferase (ptb) were deleted from Bacillus amyloliquefaciens HZ-12, and the concentrations of branched-chain short fatty acids (BCFAs) and DNJ were compared.
RESULTS
By knockout of the ptb gene, 1.01 g BCFAs kg was produced from fermented soybean by HZ-12Δptb. This was a 56% decrease compared with that of HZ-12 (2.27 g BCFAs kg). Moreover, no significant difference was found in the DNJ concentration (0.7 g kg). After further deletion of the bcd gene from HZ-12Δptb, no BCFAs was detected in fermented soybeans with HZ-12ΔptbΔbcd, while the DNJ yield decreased by 26% compared with HZ-12.
CONCLUSIONS
HZ-12Δptb had decreased BCFAs formation but also maintained the stable DNJ yield, which contributed to producing DNJ-rich products with decreased unpleasant smell.
目的
为减少1-脱氧野尻霉素（DNJ）生产过程中的难闻气味，从解淀粉芽孢杆菌HZ-12中删除亮氨酸脱氢酶（bcd）和磷酸丁酰转移酶（ptb）基因，并比较支链短链脂肪酸（BCFAs）和DNJ的浓度。
结果
通过敲除ptb基因，HZ-12Δptb从发酵大豆中产生了1.01 g/kg的BCFAs。与HZ-12（2.27 g/kg的BCFAs）相比，减少了56%。此外，DNJ浓度（0.7 g/kg）没有显著差异。从HZ-12Δptb进一步删除bcd基因后，HZ-12ΔptbΔbcd发酵大豆中未检测到BCFAs，而DNJ产量与HZ-12相比下降了26%。
结论
HZ-12Δptb减少了BCFAs的形成，但也保持了稳定的DNJ产量，这有助于生产出气味难闻程度降低且富含DNJ的产品。
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