Xu Jing, Wu Weibing, Wang Jun, Huang Chenjun, Wen Wei, Zhao Fei, Xu Xinfeng, Pan Xianglong, Wang Wei, Zhu Quan, Chen Liang
Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
Oncol Rep. 2017 Feb;37(2):1052-1058. doi: 10.3892/or.2016.5314. Epub 2016 Dec 14.
The involvement of miR-367 in lung cancer development remains unclear. In the present study, we analyzed the expression of miR-367 in tumor and adjacent tissue samples from 113 patients with non-small cell lung cancer (NSCLC) utilizing real-time PCR. miR-367 expression was significantly upregulated in the cancer tissues compared with non-cancer controls. Based on the median value of the miR-367 expression level, we divided the NSCLC patients into miR-367 high-expression and miR-367 low-expression groups. Overexpression of miR-367 was correlated with a poorer prognosis of NSCLC patients Chi-square (χ2) test showed a significant statistical correlation between tumor size, tumor stage, metastasis and miR-367 expression. Additionally, miR-367 expression was found to be negatively correlated with FBXW7 expression. Based on the above correlations, we performed a series of functional experiments to further confirm the effect of miR-367 on NSCLC. Our results indicated that miR-367 may be involved in the development and progression of NSCLC by promoting proliferation and invasion and impeding apoptosis in NSCLC cells. Furthermore, FBXW7 was identified as a potential target of miR-367, and FBXW7 silencing partially compromised the invasive, proliferative and migratory capacities in the cells with low miR-367 expression. Thus, the miR-367/FBXW7 axis may be involved in the development and progression of NSCLC and may be valuable as a therapeutic target for the treatment of human NSCLC, especially cancers with high invasive potential.
miR-367在肺癌发展中的作用仍不清楚。在本研究中，我们利用实时PCR分析了113例非小细胞肺癌（NSCLC）患者肿瘤及癌旁组织样本中miR-367的表达。与非癌对照相比，癌组织中miR-367表达显著上调。根据miR-367表达水平的中位数，我们将NSCLC患者分为miR-367高表达组和miR-367低表达组。miR-367过表达与NSCLC患者较差的预后相关。卡方（χ2）检验显示肿瘤大小、肿瘤分期、转移与miR-367表达之间存在显著的统计学相关性。此外，发现miR-367表达与FBXW7表达呈负相关。基于上述相关性，我们进行了一系列功能实验以进一步证实miR-367对NSCLC的作用。我们的结果表明，miR-367可能通过促进NSCLC细胞增殖、侵袭并抑制其凋亡而参与NSCLC的发生和发展。此外，FBXW7被确定为miR-367的潜在靶点，FBXW7沉默部分削弱了低miR-367表达细胞的侵袭、增殖和迁移能力。因此，miR-367/FBXW7轴可能参与NSCLC的发生和发展，并且可能作为治疗人类NSCLC尤其是具有高侵袭潜能癌症的治疗靶点具有重要价值。
