Filippova V N, Filanovskaia L I, Blinov M N, Ushakova E A
Vopr Med Khim. 1978 Sep-Oct;24(5):666-70.
Effect of thermic treatment and of pre-incubation with phosphoribosyl pyrophosphate on activity of AMP- and IMP-pyrophosphorylases were studied in leukocytes under chronic forms of limpho- and myeloleukoses. AMP-pyrophosphorylase from leukemia leukocytes was inactivated after heating up to 65 degrees, which, at the same time, as distinct from the enzyme of normal leukocytes, proved to be reversibly reduced after incubation of the heated cell extracts with phosphoribosyl pyrophosphate. IMP-pyrophosphorylase of leukocytes was activated at this temperature; the activation of the enzyme was 15--20% higher in leukemic leukocytes than in leukocytes of healthy donors. The data obtained demonstrate the increased thermostability of purine nucleotide pyrophosphorylase from leukemic leukocytes, which is apparently due to conformational peculiarities of the enzyme molecules.
研究了热处理以及与磷酸核糖焦磷酸预温育对慢性淋巴细胞白血病和髓细胞白血病患者白细胞中AMP-焦磷酸化酶和IMP-焦磷酸化酶活性的影响。白血病白细胞中的AMP-焦磷酸化酶在加热至65摄氏度后失活，然而，与正常白细胞的酶不同的是，加热后的细胞提取物与磷酸核糖焦磷酸温育后，该酶活性被可逆性降低。白细胞中的IMP-焦磷酸化酶在此温度下被激活；白血病白细胞中该酶的激活程度比健康供体白细胞中的高15%-20%。所获得的数据表明白血病白细胞中嘌呤核苷酸焦磷酸化酶的热稳定性增加，这显然是由于酶分子的构象特性所致。
