Kidder G W, Nolan L L, Dewey V C
J Parasitol. 1979 Aug;65(4):520-5.
The purine phosphoribosyltransferases of Crithidia fasciculata were identified and some of their properties described. The organism possesses three separate enzymes for the production of AMP, IMP, and GMP. The evidence for this comes from the observed differences in elution patterns from gel filtration columns, differences in heat sensitivity, and especially the clear separation of hypoxanthine phosphoribosyltransferase from guanine phosphoribosyltransferase by affinity chromatography on GMP-agarose. APRTase is activated most efficiently by Zn++, whereas HPRTase and GPRTase are activated most effectively by Co++. In no case did the product mononucleotides produce strong inhibition of the transferase activities.
已鉴定出纤细短膜虫的嘌呤磷酸核糖转移酶，并描述了其中一些酶的特性。该生物体拥有三种分别用于生成AMP、IMP和GMP的酶。这一结论的证据来自于凝胶过滤柱洗脱模式的观察差异、热敏感性差异，尤其是通过在GMP-琼脂糖上进行亲和层析，次黄嘌呤磷酸核糖转移酶与鸟嘌呤磷酸核糖转移酶的清晰分离。APRTase最有效地被Zn++激活，而HPRTase和GPRTase最有效地被Co++激活。在任何情况下，产物单核苷酸都不会对转移酶活性产生强烈抑制。
